產品編號 | bs-14486R-Gold |
英文名稱1 | Rabbit Anti-ECM29/Gold Conjugated antibody |
中文名稱 | 膠體金標記的ECM29蛋白抗體 |
別 名 | ECM29 homolog, proteasome accessory protein; FLJ22036; ECM29_HUMAN; KIAA0368; KIAA1962; Proteasome associated protein ECM29 homolog; RP11-386D8.2. |
規格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul(10nm 15nm 35nm) |
研究領域 | 細胞生物 泛素 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | (predicted: Human, Mouse, Rat, Pig, Cow, Horse, Sheep, ) |
產品應用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 204kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 0.4mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human ECM29 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
保存條件 | Store at 2-8 oC for 3-6 months. Avoid repeated freeze/thaw cycles. |
產品介紹 |
background: ECM29 is a 1,845 amino acid protein containing twenty-seven HEAT repeats. Localizing primarily to endoplasmic reticulum, ECM29 acts as an adapter or scaffolding protein, binding to the 26S proteasome, motor proteins, and other compartment specific proteins. The N-terminus of ECM29 binds endocytic proteins, including rabaptin, Rab11-FIP4, and VPS11, while the C-terminus binds myosins and kinesins. ECM29 may also play a role in endoplasmic reticulum (ER)-associated protein degradation (ERAD) in addition to other enhanced proteolysis. Function: ECM29 appears to be one of three proteins which associate significantly with proteasomes. ECM29 has been shown to enhance the stability of the proteasome. ECM29 deletion has been shown to produce 26S proteasomes which dissociated in the absence of ATP. It was thus suggested that ECM29 tethers the regulatory complex to the proteasome. Subcellular Location: Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Endosome. Cytoplasm ?cytoskeleton ?centrosome. Nucleus. Similarity: Contains 27 HEAT repeats. Database links: Entrez Gene: 23392 Human Entrez Gene: 230249 Mouse SwissProt: Q5VYK3 Human SwissProt: Q6PDI5 Mouse Unigene: 368255 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |