產(chǎn)品編號 | bs-10075R-BF555 |
英文名稱1 | Rabbit Anti-Nidogen2/BF555 Conjugated antibody |
中文名稱 | BF555標記的骨巢蛋白抗體 |
別 名 | NID 2; NID2; Nidogen2; Nidogen 2; Nidogen-2; Osteonidogen; NID2_HUMAN. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領域 | 細胞生物 神經(jīng)生物學 信號轉(zhuǎn)導 細胞周期蛋白 細胞粘附分子 細胞分化 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, ) |
產(chǎn)品應用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 148kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Nidogen2 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: Nidogen 2 is an adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell extracellular matrix interactions. Function: Cell adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell-extracellular matrix interactions. Subunit: Interacts with LAMA2. Interacts with COL13A1. Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane. Tissue Specificity: Heart, placenta and bone. Less in pancreas, kidney and skeletal muscle. Post-translational modifications: Highly N- and O-glycosylated. Similarity: Contains 5 EGF-like domains. Contains 5 LDL-receptor class B repeats. Contains 1 NIDO domain. Contains 1 nidogen G2 beta-barrel domain. Contains 2 thyroglobulin type-1 domains. Database links: Entrez Gene: 22795 Human Entrez Gene: 18074 Mouse Omim: 605399 Human SwissProt: Q14112 Human SwissProt: O88322 Mouse Unigene: 147697 Human Unigene: 369840 Human Unigene: 20348 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |