產品編號 | bs-0664R-Gold |
英文名稱1 | Rabbit Anti-HMGB1/Gold Conjugated antibody |
中文名稱 | 膠體金標記的高遷移率族蛋白B1抗體 |
別 名 | Amphoterin; High mobility group 1; High Mobility Group Box 1; High mobility group protein 1; High mobility group protein B1; HMG3; HMGB 1;HMGB-1; Hmgb1 protein; Nonhistone chromosomal protein HMG1; SBP 1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; HMGB1_HUMAN. |
規格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul(10nm 15nm 35nm) |
研究領域 | 腫瘤 細胞生物 免疫學 轉錄調節因子 結合蛋白 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Human, (predicted: Mouse, Rat, Cow, ) |
產品應用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 25kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 0.4mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human HMGB1 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
保存條件 | Store at 2-8 oC for 3-6 months. Avoid repeated freeze/thaw cycles. |
產品介紹 |
background: High Mobility Group Box-1 (HMGB1) is a cytokine implicated in the pathogenesis of rheumatoid arthritis (RA) and other inflammatory diseases. The cholinergic anti-inflammatory pathway, a vagus nerve dependent mechanism, inhibits HMGB1 release in experimental disease models Function: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Subunit: Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Subcellular Location: Nucleus. Chromosome. Similarity: Belongs to the HMGB family. Contains 2 HMG box DNA-binding domains. Database links: Entrez Gene: 3146 Human Entrez Gene: 100862258 Mouse Entrez Gene: 15289 Mouse Omim: 163905 Human SwissProt: P09429 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 近來的研究表明稱之為高遷移率族蛋白B-1(HMG-B1)的核內結構蛋白在核外表達時是一種有效的早期炎癥介質。 高遷移性B1組蛋白(HMGB1): 是一種核結合蛋白,在DNA重組、修復、復制和基因轉錄中起作用。HMGB1也是巨噬細胞分泌的一種介質。 此外,高遷移性B1組蛋白亦被受刺激的巨噬細胞或單核細胞主動分泌。在這一主動分泌過程中,HMGB1首先經乙酰化并由核內轉移至溶酶體內,繼而在ATP和溶血磷脂膽堿兩種分泌信號指導下轉移至細胞外。由壞死細胞被動釋放的HMGB1和炎癥細胞主動分泌的HMGB1存在分子上的差異。胞外的HMGB1可作為細胞因子參與信號傳導,因為它既可識別Toll 樣受體(TLR)家族的一些成員,又能與識別晚期糖基化終末產物受體(RAGE)相作用。 HMGB1能啟動炎癥反應,包括產生多種細胞因子、對某些干細胞產生趨化作用、誘導血管粘附分子、削弱腸上皮細胞的功能等。 |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |