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Rabbit Anti-His tag/BF350 Conjugated antibody (bs-0287R-BF350)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產(chǎn)品編號 bs-0287R-BF350
英文名稱1 Rabbit Anti-His tag/BF350 Conjugated antibody
中文名稱 BF350標(biāo)記的聚組氨酸抗體標(biāo)簽抗體
別    名 HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.  
規(guī)格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
產(chǎn)品類型 標(biāo)簽抗體 
研究領(lǐng)域 腫瘤  細(xì)胞生物  免疫學(xué)  染色質(zhì)和核信號  信號轉(zhuǎn)導(dǎo)  合成與降解  結(jié)合蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Recombinant protein
產(chǎn)品應(yīng)用
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 N/AkDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 Synthetic peptide: HHHHHH conjugated to KLH
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

標(biāo)簽抗體
蛋白質(zhì)純化的新途徑─應(yīng)用His-Tag抗體檢測His-Tag融合蛋白

1) 6xHis與不同的Sepharose純化填料結(jié)合(捆綁),可不受構(gòu)象的影響,在天然或變形的條件下可進(jìn)行進(jìn)一步純化。
2) 可在溫和的洗脫液環(huán)境中,其結(jié)合、洗滌及洗脫步驟不會對蛋白結(jié)構(gòu)產(chǎn)生影響。
3) 6xHis標(biāo)簽比普通(Tag)要小得多,經(jīng)純化后的蛋白可直接進(jìn)行下游操作。可用于任何表達(dá)系統(tǒng)(包括:
細(xì)菌,桿狀病毒和哺乳動物)
4) 6xHis標(biāo)簽在生理溶液pH下不帶電荷,其標(biāo)簽不會干涉重組蛋白的結(jié)構(gòu)和功能, 6xHis不會影響蛋白分泌
5) 6xHis標(biāo)簽免疫原性差,重組蛋白可以不必除去標(biāo)簽而直接作為抗原進(jìn)行免疫



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