產品編號 | bs-3940R-HRP |
英文名稱1 | Rabbit Anti-G protein beta 4/HRP Conjugated antibody |
中文名稱 | 辣根過氧化物酶標記的G蛋白β4抗體/鳥嘌呤核苷酸結合蛋白β亞基4 |
別 名 | G protein beta 4 subunit; GNB 4; GNB4; Guanine nucleotide binding protein (G protein) beta polypeptide 4; Guanine nucleotide binding protein beta 4 subunit; Guanine nucleotide binding protein beta polypeptide 4; Guanine nucleotide binding protein beta subunit 4; Guanine nucleotide binding protein subunit beta 4; Transducin beta chain 4; GBB4_HUMAN. |
規格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領域 | 腫瘤 細胞生物 免疫學 轉錄調節因子 結合蛋白 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | (predicted: Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, ) |
產品應用 | WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 37kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human G protein beta 4 (52-90aa) |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產品介紹 |
background: Guanine nucleotide binding proteins (G proteins) are membrane associated, heterotrimeric proteins composed of three subunits: alpha, beta and gamma. The G protein beta subunit assumes a barrel shaped beta propeller structure containing WD40 repeats preceded by an N terminal alpha helix. The beta subunit forms a stable dimer with the gamma subunit. The alpha subunit only contacts the beta subunit in the dimer, lying on the opposite face from the gamma subunit. G proteins and their receptors (GPCRs) regulate systems as diverse as sensory perception, cell growth and hormonal regulation. Function: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. Subunit: G proteins are composed of 3 units, alpha, beta and gamma. Tissue Specificity: Strongly expressed in lung and placenta, whereas it is weakly expressed in brain and heart. Similarity: Belongs to the WD repeat G protein beta family. Contains 7 WD repeats. Database links: Entrez Gene: 59345 Human Entrez Gene: 14696 Mouse Omim: 610863 Human SwissProt: Q9HAV0 Human SwissProt: P29387 Mouse SwissProt: Q3THF3 Mouse SwissProt: Q3TY18 Mouse SwissProt: Q3U2F9 Mouse Unigene: 173030 Human Unigene: 139192 Mouse Unigene: 10844 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |