產(chǎn)品編號 | bs-2652R-FITC |
英文名稱1 | Rabbit Anti-SPHK1/FITC Conjugated antibody |
中文名稱 | FITC標記的鞘氨醇激酶1抗體 |
別 名 | sphingosine kinase 1; SK 1; SK1; SPHK 1; SPHK; SPK; SPK 1; SPK1; Sphk1; SPHK1_HUMAN. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領域 | 腫瘤 細胞生物 信號轉(zhuǎn)導 細胞凋亡 激酶和磷酸酶 細胞分化 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Mouse, (predicted: Human, Rat, Dog, Cow, Rabbit, ) |
產(chǎn)品應用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 42-52kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human SPHK1 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] Function: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Tissue Specificity: Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. Similarity: Contains 1 DAGKc domain. Database links: Entrez Gene: 8877 Human Entrez Gene: 20698 Mouse Omim: 603730 Human SwissProt: Q9NYA1 Human SwissProt: Q8CI15 Mouse Unigene: 68061 Human Unigene: 20944 Mouse Unigene: 18522 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. SPHK1參與鞘氨醇代謝、G蛋白偶聯(lián)受體蛋白信號轉(zhuǎn)導途徑,有抗細胞凋亡的作用,可被蛋白激酶C激活,參與細胞內(nèi)信號轉(zhuǎn)導級聯(lián)、鈣介導信號轉(zhuǎn)導、調(diào)控細胞生長遷移、調(diào)控血管生成及成纖維細胞增殖。 |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |