| 產品編號 | bs-43034R |
| 英文名稱 | Rabbit Anti-HAVCR2/TIM-3 antibody |
| 中文名稱 | T淋巴細胞膜蛋白3(CD366)抗體 |
| 別 名 | TIM-3; Tim 3; TIM3; TIMD-3; TIMD3; HAVR2_HUMAN; Hepatitis A virus cellular receptor 2; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; CD366; |
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Specific References (1) | bs-43034R has been referenced in 1 publications.
[IF=4.047] Xie Hongbing. et al. Tim-3 downregulation by Toxoplasma gondii infection contributes to decidual dendritic cell dysfunction. PARASITE VECTOR. 2022 Dec;15(1):1-17 WB ; Human.
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| 研究領域 | 細胞生物 免疫學 淋巴細胞 t-淋巴細胞 細胞膜蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse |
| 產品應用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 29kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human TIM-3 protein: 22-200/301 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011] SWISS: Q8TDQ0 Gene ID: 84868 |
| 產品圖片 |
Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Lymph node tissue lysates
Lane 3: Mouse Lung tissue lysates
Primary: Anti-HAVCR2 (bs-43034R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 47 kDa
Measured by its binding ability in a indirect ELISA. Immobilized Human TIM-3 protein, HisTag (Cat. bs-43034P) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Human HAVCR2 Antibody, the EC50 is 959.2 ng/mL.
Measured by its binding ability in a indirect ELISA. Immobilized Human TIM-3 protein, His Tag (Cat. bs-43034P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Human HAVCR2 Antibody, the EC50 is 533.1 ng/mL.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
