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Mouse Anti-CYP1A2  antibody (bsm-51530M)  
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產品編號 bsm-51530M
英文名稱 Mouse Anti-CYP1A2  antibody
中文名稱 CYP1A2單克隆抗體
別    名 Cytochrome P450 1A2; cytochrome P450, family 1, subfamily a, polypeptide 2; Cytochrome P450 family 1 polypeptide 2; Cytochrome P450 subfamily I aromatic compound inducible polypeptide 2; Cyp1a2; CYPD45; P-450d; RATCYPD45; CP12; CP 12; P3-450; Aryl hydrocarbon hydroxylase; Dioxin inducable P3 45; Flavoprotein linked monooxygenase; Microsomal monooxygenase; P450 4; P450 form 4; P450 P3; P450(PA); Xenobiotic monooxygenase; P450 1A2.  
研究領域 細胞生物  神經生物學  合成與降解  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號 B7F5
交叉反應 Human
產品應用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 56kDa
細胞定位 細胞漿 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CYP1A2: 201-300/513 
亞    型 IgG3
純化方法 affinity purified by Protein G
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu region. [provided by RefSeq].sequences flanked by direct repeats in the 3' untranslated.

Function:
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin.

Subcellular Location:
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue Specificity:
Liver.

Similarity:
Belongs to the cytochrome P450 family.

SWISS:
P05177

Gene ID:
1544

Database links:

Entrez Gene: 1544 Human

SwissProt: P05177 Human



產品圖片
Sample: Lane 1: SK-BR-3 cell lysates Primary: Anti-CYP1A2 (bsm-51530M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 60 kD
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