產品編號 | bsm-51505M |
英文名稱 | Mouse Anti-THAP11 antibody |
中文名稱 | THAP11單克隆抗體 |
別 名 | CTG B43a; CTG B45d; HRIHFB2206; RONIN; THA11_HUMAN; THAP 11; THAP domain containing 11; THAP domain-containing protein 11. |
研究領域 | 細胞生物 轉錄調節因子 結合蛋白 表觀遺傳學 |
抗體來源 | Mouse |
克隆類型 | Monoclonal |
克 隆 號 | S26F1 |
交叉反應 | Human,Mouse (predicted: Cow) |
產品應用 | WB=1:500-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 37kDa |
細胞定位 | 細胞核 細胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human THAP11: 151-250 |
亞 型 | IgM |
純化方法 | affinity purified by Protein G |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
The protein encoded by this gene contains a THAP domain, which is a conserved DNA-binding domain that has striking similarity to the site-specific DNA-binding domain (DBD) of Drosophila P element transposases. [provided by RefSeq, Jul 2008] Function: Transcriptional repressor that plays a central role for embryogenesis and the pluripotency of embryonic stem (ES) cells. Sequence-specific DNA-binding factor that represses gene expression in pluripotent ES cells by directly binding to key genetic loci and recruiting epigenetic modifiers. Subcellular Location: Nucleus. Cytoplasm. May be regulated by shuttling of the protein between the cytoplasm and nucleus. Similarity: Belongs to the THAP11 family. Contains 1 THAP-type zinc finger. SWISS: Q96EK4 Gene ID: 57215 Database links: Entrez Gene: 57215 Human Entrez Gene: 59016 Mouse
SwissProt: Q96EK4 Human SwissProt: Q9JJD0 Mouse
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產品圖片 |
Sample:
Lane 1: HepG2 cell lysates
Primary: Anti-THAP11 (bsm-51505M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 32 kD
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |