| 產品編號 | bsm-52830R |
| 英文名稱 | Rabbit Anti-NQO1 antibody |
| 中文名稱 | 醌氧化還原酶重組兔單抗 |
| 別 名 | Azoreductase; Cytochrome b 5 reductase; DHQU; DIA 4; DIA4; Diaphorase (NADH/NADPH) (cytochrome b 5 reductase); Diaphorase (NADH/NADPH) (cytochrome b-5 reductase); Diaphorase (NADH/NADPH) (cytochrome b-5 reductase); Diaphorase (NADH/NADPH); Diaphorase 4; Dioxin inducible 1; DT diaphorase; DT-diaphorase; DTD; Menadione reductase; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase quinone 1; NAD(P)H menadione oxidoreductase 1 dioxin inducible; NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; NMOR 1; NMOR I; NMOR1; NMORI; NQO 1; NQO1_HUMAN; Phylloquinone reductase; Phylloquinone reductase; QR 1; QR1; Quinone reductase 1; Quinone reductase 1. |
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Specific References (1) | bsm-52830R has been referenced in 1 publications.
[IF=1.789] Zeba Farooqui . et al. Nrf2 inhibition induces oxidative stress, renal inflammation and hypertension in mice. Clin Exp Hypertens. 2021;43(2):175-180 WB ; Mouse.
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| 研究領域 | 腫瘤 信號轉導 合成與降解 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 8T5 |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-3000,Flow-Cyt=1:50,ICC/IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 31kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NQO1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]. Function: The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. Subunit: Homodimer. Subcellular Location: Cytoplasm. Similarity: Belongs to the NAD(P)H dehydrogenase (quinone) family. SWISS: P15559 Gene ID: 1728 Database links: Entrez Gene: 1728 Human Entrez Gene: 18104 Mouse Omim: 125860 Human SwissProt: P15559 Human SwissProt: Q64669 Mouse Unigene: 406515 Human Unigene: 252 Mouse Unigene: 11234 Rat |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with NQO1 monoclonal antibody, unconjugated (bsm-52830R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-NQO1 antibody (bsm-52830R)
Dilution: 1:100;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1:1000.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
