產(chǎn)品編號(hào) | bs-24134R |
英文名稱 | Rabbit Anti-RANBP3L antibody |
中文名稱 | Ran結(jié)合蛋白3樣蛋白抗體 |
別 名 | Ran-binding protein 3-like; Ranbp3l; RNB3L_MOUSE. |
研究領(lǐng)域 | 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 結(jié)合蛋白 |
抗體來(lái)源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Mouse |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 52kDa |
細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from mouse RANBP3L: 331-430/491 |
亞 型 | |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
RANBP3L (RAN Binding Protein 3 Like) is a Protein Coding gene. An important paralog of this gene is RANBP3. Function: Nuclear export factor for BMP-specific SMAD1/5/8 that plays a critical role in terminating BMP signaling and regulating mesenchymal stem cell differentiation by blocking osteoblast differentiation to promote myogenic differention. Directly recognizes dephosphorylated SMAD1/5/8 and mediates their nuclear export in a Ran-dependent manner. Subunit: Interacts with SMAD1, SMAD5 and SMAD8. SWISS: Q6PDH4 Gene ID: 223332 Database links: Entrez Gene: 202151 Human Entrez Gene: 223332 Mouse SwissProt: Q86VV4 Human SwissProt: Q6PDH4 Mouse Unigene: 729817 Human |
產(chǎn)品圖片 |
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-RANBP3L (bs-24134R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Sample:
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-RANBP3L (bs-24134R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RANBP3L) Polyclonal Antibody, Unconjugated (bs-24134R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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