| 產品編號 | bs-23048R |
| 英文名稱 | Rabbit Anti-CAPN1 antibody |
| 中文名稱 | 鈣蛋白酶1抗體 |
| 別 名 | Calcium activated neutral proteinase 1; Calpain1; Calpain-1; Calpain 1; Calpain 1 catalytic subunit; Calpain large polypeptide L1; Calpain mu type; CANP 1; CANP; CANP1; CANPL1; CAPN 1; Cell proliferation inducing gene 30 protein; Cell proliferation inducing protein 30; Micromolar calpain; muCANP; muCL; Ca2 activated neutral protease; Calcium activated neutral proteinase small subunit; Calcium dependent protease small subunit 1; Calcium-activated neutral proteinase 1; Calpain 1 (mu/I) large subunit; Calpain 1 catalytic subunit; Calpain 1 large subunit; Calpain Large Polypeptide L1; Calpain Large Polypeptide L1; Calpain mu type; Calpain mu-type; Calpain regulatory subunit; Calpain small subunit 1; Calpain-1 catalytic subunit; Calpain-1 large subunit; Calpain1; CAN1_HUMAN. |
| 研究領域 | 細胞生物 信號轉導 細胞凋亡 泛素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human,Mouse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 78kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Calpain 1: 521-620/713 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] Function: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Subunit: Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Subcellular Location: Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding. Tissue Specificity: Ubiquitous. Post-translational modifications: Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms. Similarity: Belongs to the peptidase C2 family. Contains 1 calpain catalytic domain. Contains 4 EF-hand domains. SWISS: O35350 Gene ID: 12333 Database links: Entrez Gene: 12333 Mouse Entrez Gene: 823 Human Omim: 114220 Human SwissProt: P07384 Human SwissProt: O35350 Mouse Unigene: 502842 Human Unigene: 6221 Mouse Unigene: 6037 Rat 鈣蛋白酶(calpain)是一族鈣離子依賴性的、水解半胱氨酸的蛋白酶,廣泛存在于全身各臟器組織中。鈣蛋白酶參與不同細胞過程,包括細胞骨架和細胞膜附著的構型重建、不同的信號轉導途徑和細胞凋亡等. |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CAN1) Polyclonal Antibody, Unconjugated (bs-23048R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
