| 產品編號 | bs-21291R |
| 英文名稱 | Rabbit Anti-RBM19 antibody |
| 中文名稱 | RNA結合蛋白19抗體 |
| 別 名 | 1200009A02Rik; AV302714; NPO; Probable RNA-binding protein 19; RBM19; RBM19_HUMAN; RNA-binding motif protein 19. |
| 研究領域 | 信號轉導 轉錄調節因子 結合蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rabbit,Pig,Sheep,Cow,Zebrafish,Dog,Horse) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 108kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RBM19: 1-100/960 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a nucleolar protein that contains six RNA-binding motifs. The encoded protein may be involved in regulating ribosome biogenesis. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Apr 2009] Function: Plays a role in embryo pre-implantation development. Subcellular Location: Nucleus > nucleolus. Nucleus > nucleoplasm. Cytoplasm. Chromosome. In discrete foci distributed throughout the cytoplasm and nucleoplasm during the 4 to 8 cell stages and the morula stage, but not in the periphery of the nucleolar precursor body (NPB). During blastocyst development, becomes increasingly localized to the nucleolus and less to the cytoplasm. At the late blastocyst stage, localized predominantly in the nucleolus. Localized in the nucleolus during interphase and to the perichromosomal sheath during mitosis. Does not colocalize in the cytoplasm with GW182 in P-bodies. May translocate to the nucleolus upon early embryonic development (By similarity). Colocalizes with NPM1 during interphase. By late prophase, metaphase, anaphase and telophase, associates with the chromosome periphery. By telophase localizes to NPB. Tissue Specificity: Expressed in the crypts of Lieberkuhn of the intestine and in intestinal neoplasia (at protein level). Similarity: Belongs to the RRM MRD1 family. Contains 6 RRM (RNA recognition motif) domains. SWISS: Q9Y4C8 Gene ID: 9904 Database links: Entrez Gene: 9904 Human Entrez Gene: 74111 Mouse SwissProt: Q9Y4C8 Human SwissProt: Q8R3C6 Mouse Unigene: 7482 Human |
| 產品圖片 |
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti- RBM19 (bs-21291R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 108 kD
Observed band size: 108 kD
Sample:
K562(Human) Cell Lysate at 30 ug
Primary: Anti- RBM19 (bs-21291R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 108 kD
Observed band size: 108 kD
Sample:
HL60(Human) Cell Lysate at 30 ug
Primary: Anti- RBM19 (bs-21291R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:108 kD
Observed band size: 110 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
