產品編號 | bs-21146R |
英文名稱 | Rabbit Anti-RALGAPB/p170 antibody |
中文名稱 | P170蛋白抗體 |
別 名 | dJ1100H13.1; KIAA1219; p170; Ral GTPase activating protein, beta subunit (non-catalytic); Ral GTPase-activating protein subunit beta; RalGAPbeta; RP5-1100H13.1. |
研究領域 | 細胞生物 信號轉導 結合蛋白 G蛋白信號 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 167kDa |
細胞定位 | 細胞核 細胞漿 細胞膜 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human RALGAPB/p170: 821-920/1494 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
Non-catalytic subunit of the heterodimeric RalGAP1 and RalGAP2 complexes which act as GTPase activators for the Ras-like small GTPases RALA and RALB SWISS: Q86X10 Gene ID: 57148 Database links: Entrez Gene: 57148 Human Entrez Gene: 228850 Mouse SwissProt: Q86X10 Human SwissProt: Q8BQZ4 Mouse |
產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RALGAPB/p170) Polyclonal Antibody, Unconjugated (bs-21146R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RALGAPB/p170) Polyclonal Antibody, Unconjugated (bs-21146R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |