產品編號 | bs-12781R |
英文名稱 | Rabbit Anti-VRTN antibody |
中文名稱 | VRTN蛋白抗體 |
別 名 | C14orf115; Vertnin; vrtn; VRTN_HUMAN. |
研究領域 | 結合蛋白 表觀遺傳學 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Cow) |
產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 78kDa |
細胞定位 | 細胞核 細胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human VRTN: 221-320/702 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
VRTN (Vertebrae Development Associated) is a Protein Coding gene. GO annotations related to this gene include sequence-specific DNA binding and transposase activity. Similarity: Belongs to the vertnin family. SWISS: Q9H8Y1 Gene ID: 55237 Database links: Entrez Gene: 55237 Human SwissProt: Q9H8Y1 Human Unigene: 578167 Human |
產品圖片 |
Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-VRTN (bs-12781R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 78 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VRTN) Polyclonal Antibody, Unconjugated (bs-12781R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
|
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |