產品編號 | bs-12641R |
英文名稱 | Rabbit Anti-SMPD4 antibody |
中文名稱 | 鞘磷脂磷酸二酯酶4抗體 |
別 名 | EC 3.1.4.12; EC=3.1.4.123; FLJ20297; FLJ20756; KIAA1418; NET 13; NET13; Neutral sphingomyelinase 3; Neutral sphingomyelinase III; NSMA3_HUMAN; nSMase 3; nSMase-3; nSMase3; OTTHUMP00000162391; OTTHUMP00000190862; SKNY protein; smpd4; Sphingomyelin phosphodiesterase 4; Sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase 3). |
研究領域 | 細胞生物 神經生物學 轉運蛋白 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Dog,Horse) |
產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 93kDa |
細胞定位 | 細胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human SMPD4: 201-300/827 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
Sphingomyelinases (EC 3.1.4.12), such as SMPD4, catalyze the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide (Krut et al., 2006 [PubMed 16517606]).[supplied by OMIM, Mar 2008] Function: Catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. Subunit: Associates with spliceosomes. Associates with U4/U5/U6 tri-snRNP and with U2 snRNP. Subcellular Location: Endoplasmic reticulum membrane. Golgi apparatus membrane. Tissue Specificity: Widely expressed, with highest levels in heart and skeletal muscle. Similarity: Belongs to the SMN family. Contains 1 Tudor domain. SWISS: Q9NXE4 Gene ID: 55627 Database links: Entrez Gene: 55627 Human Entrez Gene: 77626 Mouse Omim: 610457 Human SwissProt: Q9NXE4 Human SwissProt: Q6ZPR5 Mouse Unigene: 516450 Human |
產品圖片 |
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-SMPD4 (bs-12641R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 93 kD
Observed band size: 96 kD
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SMPD4) Polyclonal Antibody, Unconjugated (bs-12641R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |