產(chǎn)品編號 | bs-19821R |
英文名稱 | Rabbit Anti-SLC32A1 antibody |
中文名稱 | 溶質(zhì)載體家族蛋白32成員A1抗體 |
別 名 | bA122O1.1; GABA and glycine transporter; hVIAAT; SLC32A 1; Slc32a1; solute carrier family 32 (GABA vesicular transporter) member 1; Solute carrier family 32 member 1; Vesicular GABA Amino Acid Transporter; Vesicular GABA transporter; Vesicular inhibitory amino acid transporter; VGAT; VIAAT; VIAAT_HUMAN. |
研究領(lǐng)域 | 細(xì)胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)運蛋白 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Mouse,Rat (predicted: Human,Rabbit,Pig,Cow) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 57kDa |
細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human SLC30A9: 351-450/525 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II. [provided by RefSeq, Jul 2008] Function: Involved in the uptake of GABA and glycine into the synaptic vesicles. Subcellular Location: Cytoplasmic vesicle membrane. Tissue Specificity: Retina. Expressed throughout the horizontal cells or more specifically at the terminals. Similarity: Belongs to the amino acid/polyamine transporter 2 family. SWISS: Q9H598 Gene ID: 140679 Database links: Entrez Gene: 140679 Human Entrez Gene: 22348 Mouse SwissProt: Q9H598 Human SwissProt: O35633 Mouse Unigene: 179080 Human Unigene: 143404 Mouse Unigene: 413854 Mouse Unigene: 10846 Rat
|
產(chǎn)品圖片 |
Sample:heart (Mouse) Lysate at 40 ug
Primary: Anti-SLC32A1(bs-19821R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 57kD
Observed band size: 60kD
Sample:Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-SLC32A1(bs-19821R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 57kD
Observed band size: 57kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SLC32A1 Polyclonal Antibody, Unconjugated(bs-19821R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|