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Rabbit Anti-RBM47  antibody (bs-19774R)  
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產品編號 bs-19774R
英文名稱 Rabbit Anti-RBM47  antibody
中文名稱 RNA結合蛋白47抗體
別    名 NET18; Rbm47; Rbm47 RNA binding motif protein 47; RBM47_HUMAN; RNA binding motif protein 47; RNA binding protein 47; RNA-binding motif protein 47; RNA-binding protein 47.  
研究領域 結合蛋白  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Rat (predicted: Mouse,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human RBM47: 351-450/593 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹
產品圖片
Sample: Lane 1: Human A431 cell lysates Lane 2: Human A549 cell lysates Primary: Anti-RBM47 (bs-19774R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 , 57 kDa Observed band size: 62 kDa
Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RBM47) Polyclonal Antibody, Unconjugated (bs-19774R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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