| 產(chǎn)品編號 | bs-19605R |
| 英文名稱 | Rabbit Anti-SDPR antibody |
| 中文名稱 | 磷脂酰絲氨酸結(jié)合蛋白SDPR抗體 |
| 別 名 | Cavin 2; Cavin-2; Phosphatidylserine binding protein; Phosphatidylserine-binding protein; PS p68; PS-p68; Sdpr; SDPR_HUMAN; SDR; Serum deprivation response; Serum deprivation response protein; Serum deprivation-response protein. |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導 激酶和磷酸酶 結(jié)合蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Cow) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 47kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SDPR: 231-330/425 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a calcium-independent phospholipid-binding protein whose expression increases in serum-starved cells. This protein is a substrate for protein kinase C (PKC) phosphorylation and recruits polymerase I and transcript release factor (PTRF) to caveolae. Removal of this protein causes caveolae loss and its over-expression results in caveolae deformation and membrane tubulation.[provided by RefSeq, Sep 2009] Function: May play a role in targeting PRKCA to caveolae. Subcellular Location: Cytoplasm > cytosol. Membrane > caveola. Colocalizes with CAV1 to caveolae. Tissue Specificity: Highly expressed in heart and lung, and expressed at lower levels in brain, kidney, liver, pancreas, placenta, and skeletal muscle. Post-translational modifications: Phosphorylated on Ser residues. Similarity: Belongs to the PTRF/SDPR family. SWISS: O95810 Gene ID: 8436 Database links: Entrez Gene: 8436 Human Entrez Gene: 20324 Mouse Omim: 606728 Human SwissProt: O95810 Human SwissProt: Q63918 Mouse Unigene: 26530 Human Unigene: 480575 Mouse Unigene: 203035 Rat |
| 產(chǎn)品圖片 |
Protein: heart(mouse) lysate at 40ug;
Primary: rabbit Anti-SDPR (bs-19605R) at 1:300;
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SDPR) Polyclonal Antibody, Unconjugated (bs-19605R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
