產品編號 | bs-19364R |
英文名稱 | Rabbit Anti-SAE1 antibody |
中文名稱 | 泛素樣蛋白SUMO1激活酶抗體 |
別 名 | Activator of SUMO1; AOS 1; AOS1; FLJ3091; HSPC 140; HSPC140; SAE 1; Sae1; SAE1_HUMAN; Sentrin/SUMO activating protein AOS 1; Sentrin/SUMO activating protein AOS1; SUA 1; SUA1; SUMO 1 activating enzyme E1 N subunit; SUMO 1 activating enzyme subunit 1; SUMO-activating enzyme subunit 1; SUMO1 activating enzyme E1 N subunit; SUMO1 activating enzyme subunit 1; Ubiquitin like 1 activating enzyme E1A; Ubiquitin like protein SUMO 1 activating enzyme; Ubiquitin like protein SUMO1 activating enzyme; Ubiquitin-like 1-activating enzyme E1A; UBL E1A; UBLE 1A; UBLE1A. |
研究領域 | 細胞生物 泛素 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Mouse (predicted: Human,Rat) |
產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 29kDa |
細胞定位 | 細胞核 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human SAE1,Isoform 2: 1-100/346 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010] Subunit: Heterodimer of SAE1 and UBA2/SAE2. The heterodimer corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1. Interacts with UBE2I. Subcellular Location: Nucleus. Tissue Specificity: Expression level increases during S phase and drops in G2 phase (at protein level). Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the ubiquitin-activating E1 family. SWISS: Q9UBE0 Gene ID: 10055 Database links: Entrez Gene: 10055 Human Entrez Gene: 56459 Mouse Omim: 613294 Human SwissProt: Q9UBE0 Human SwissProt: Q9R1T2 Mouse Unigene: 515500 Human Unigene: 258530 Mouse Unigene: 9014 Rat |
產品圖片 |
Sample: brain (mouse) Lysate at 40 ug
Primary: Anti-SAE1(bs-19364R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD
|
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |