| 產品編號 | bs-19273R |
| 英文名稱 | Rabbit Anti-BarX2 antibody |
| 中文名稱 | BarX2蛋白抗體 |
| 別 名 | BarH like homeobox 2; BarX 2; BARX homeobox; BARX homeobox 2; Barx2; BARX2_HUMAN; Homeobox protein BarH like 2; Homeobox protein BarH-like 2; MGC133368; MGC133369. |
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Specific References (1) | bs-19273R has been referenced in 1 publications.
[IF=5.682] Ling Zuo. et al. Circular RNA circSHPRH inhibits the malignant behaviors of bladder cancer by regulating the miR-942/BARX2 pathway. Aging-Us. 2022 Feb 28; 14(4): 1891–1909 WB ; Human.
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| 研究領域 | 腫瘤 轉錄調節因子 細胞粘附分子 結合蛋白 細胞骨架 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Sheep,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 31kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BarX2: 81-180/279 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a member of the homeobox transcription factor family. A highly related protein in mouse has been shown to influence cellular processes that control cell adhesion and remodeling of the actin cytoskeleton in myoblast fusion and chondrogenesis. The encoded protein may also play a role in cancer progression. [provided by RefSeq, Jul 2008] Function: Transcription factor. Binds optimally to the DNA consensus sequence 5'-YYTAATGRTTTTY-3'. May control the expression of neural adhesion molecules such as L1 or Ng-CAM during embryonic development of both the central and peripherical nervous system. May be involved in controlling adhesive processes in keratinizing epithelia. Subcellular Location: Nucleus. Tissue Specificity: Highly expressed in adult salivary gland and at much lower levels in mammary gland, kidney and placenta. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the BAR homeobox family. Contains 1 homeobox DNA-binding domain. SWISS: Q9UMQ3 Gene ID: 8538 Database links: Entrez Gene: 8538 Human Entrez Gene: 12023 Mouse Omim: 604823 Human SwissProt: Q9UMQ3 Human SwissProt: O08686 Mouse Unigene: 591944 Human Unigene: 261044 Mouse |
| 產品圖片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-BarX2 (bs-19273R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BarX2) Polyclonal Antibody, Unconjugated (bs-19273R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BarX2) Polyclonal Antibody, Unconjugated (bs-19273R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
