產品編號 | bs-18454R |
英文名稱 | Rabbit Anti-LY6G5C/C6orf20 antibody |
中文名稱 | 6號染色體開放閱讀框20抗體 |
別 名 | C6orf20; G5C; LY65C_HUMAN; LY6G5C; LY6G5CA; LY6G5CB; Lymphocyte antigen 6 complex locus protein G5c; NG33. |
研究領域 | 細胞生物 免疫學 淋巴細胞 t-淋巴細胞 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Human |
產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 12kDa |
細胞定位 | 分泌型蛋白 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human LY6G5C/C6orf20: 61-130/150 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
LY6G5C belongs to a cluster of leukocyte antigen-6 (LY6) genes located in the major histocompatibility complex (MHC) class III region on chromosome 6. Members of the LY6 superfamily typically contain 70 to 80 amino acids, including 8 to 10 cysteines. Most LY6 proteins are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor that is directly involved in signal transduction (Mallya et al., 2002 [PubMed 12079290]).[supplied by OMIM, Mar 2008] Function: May have a role in hematopoietic cell differentiation. Subcellular Location: Secreted. Tissue Specificity: Detected in T-cell lines and fetal and adult lung. Post-translational modifications: N-glycosylated. Similarity: Contains 1 UPAR/Ly6 domain. SWISS: Q5SRR4 Gene ID: 80741 Database links: Entrez Gene: 80741 Human Omim: 610434 Human SwissProt: Q5SRR4 Human Unigene: 25738 Human |
產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Human stomach cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LY6G5C/C6orf20) Polyclonal Antibody, Unconjugated (bs-18454R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LY6G5C/C6orf20) Polyclonal Antibody, Unconjugated (bs-18454R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |