| 產品編號 | bs-17567R |
| 英文名稱 | Rabbit Anti-OTUD6B antibody |
| 中文名稱 | OTUD6B蛋白抗體 |
| 別 名 | CGI-77; DUBA-5; DUBA5; OTU domain containing protein 6B; OTU domain-containing protein 6B; OTU6B_HUMAN; OTUD6B. |
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Specific References (1) | bs-17567R has been referenced in 1 publications.
[IF=3.249] Zhongqun Wang. et al. Loss of OTUD6B Stimulates Angiogenesis and Promotes Diabetic Atherosclerosis. DIABET METAB SYND OB. 2022 Sep;15:3027-3038 IF, WB ; Mouse.
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| 研究領域 | 腫瘤 細胞生物 細胞周期蛋白 b-淋巴細胞 表觀遺傳學 泛素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rabbit,Dog,Saccharomyces cerevisiae) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human OTUD6B: 51-150/293 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a member of the ovarian tumor domain (OTU)-containing subfamily of deubiquitinating enzymes. Deubiquitinating enzymes are primarily involved in removing ubiquitin from proteins targeted for degradation. This protein may function as a negative regulator of the cell cycle in B cells. [provided by RefSeq, Nov 2013] Similarity: Contains 1 OTU domain. SWISS: Q8N6M0 Gene ID: 51633 Database links: Entrez Gene: 51633 Human Entrez Gene: 72201 Mouse Omim: 612021 Human SwissProt: Q8N6M0 Human SwissProt: Q8K2H2 Mouse Unigene: 30532 Human Unigene: 387724 Mouse |
| 產品圖片 |
Sample:
Jurkat(Mouse) Cell Lysate at 40 ug
Primary: Anti-OTUD6B (bs-17567R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OTUD6B) Polyclonal Antibody, Unconjugated (bs-17567R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
