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Rabbit Anti-Histone H1.X  antibody (bs-17424R)  
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產品編號 bs-17424R
英文名稱 Rabbit Anti-Histone H1.X  antibody
中文名稱 組蛋白H1家族X抗體
別    名 H1 histone family member X; Histone H1fx; H1FX; H1X_HUMAN; H1X; Histone H1x; MGC15959; MGC8350.  
研究領域 細胞生物  表觀遺傳學  泛素  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Mouse (predicted: Rat,Pig,Sheep,Cow,Dog)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 22kDa
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Histone H1.X: 21-120/213 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H1 family. [provided by RefSeq, Jul 2008]

Function:
Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures.

Subcellular Location:
NuclearC. hromosome.

Tissue Specificity:
Expressed ubiquitously.

Post-translational modifications:
Citrullination at Arg-62 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance (By similarity).

Similarity:
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain.

SWISS:
Q92522

Gene ID:
8971

Database links:

Entrez Gene: 8971 Human

Entrez Gene: 243529 Mouse

Omim: 602785 Human

SwissProt: Q92522 Human

SwissProt: Q80ZM5 Mouse

Unigene: 33796 Mouse



產品圖片
Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-Histone H1.X (bs-17424R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 22 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H1.X) Polyclonal Antibody, Unconjugated (bs-17424R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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