| 產品編號 | bs-10578R |
| 英文名稱 | Rabbit Anti-WASF3 antibody |
| 中文名稱 | Verprolin同源結構域包含蛋白3抗體 |
| 別 名 | KIAA0900; Protein WAVE-3; Protein WAVE3; SCAR3; Verprolin homology domain containing protein 3; Verprolin homology domain-containing protein 3; WASF3; WASF3_HUMAN; WASP family protein member 3; WAVE3; Wiskott Aldrich syndrome protein family member 3; Wiskott-Aldrich syndrome protein family member 3. |
| 研究領域 | 細胞生物 信號轉導 結合蛋白 G蛋白偶聯受體 細胞骨架 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Sheep,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 55kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human WASF3: 21-120/502 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Function: Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. Subunit: Binds actin and the Arp2/3 complex. Subcellular Location: Cytoplasm, cytoskeleton. Tissue Specificity: Expressed in ovary and brain. Post-translational modifications: Phosphorylation by ABL1 promotes lamellipodia formation and cell migration. Similarity: Belongs to the SCAR/WAVE family. Contains 1 WH2 domain. SWISS: Q9UPY6 Gene ID: 10810 Database links: Entrez Gene: 10810 Human Entrez Gene: 245880 Mouse Omim: 605068 Human SwissProt: Q9UPY6 Human SwissProt: Q8VHI6 Mouse Unigene: 635221 Human Unigene: 472750 Mouse |
| 產品圖片 |
Sample: ovary (Mouse) Lysate at 40 ug
Primary: Anti-WASF3(bs-10578R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample:
Lane 1: Mouse Cerebellum tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Stomach tissue lysates
Lane 4: Mouse Heart tissue lysates
Lane 5: Rat Cerebellum tissue lysates
Lane 6: Rat Cerebrum tissue lysates
Lane 7: Rat Stomach tissue lysates
Primary: Anti-WASF3 (bs-10578R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 52 kDa
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RWASF3) Polyclonal Antibody, Unconjugated (bs-10578) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
