| 產品編號 | bs-16694R |
| 英文名稱 | Rabbit Anti-IRAK1BP1 antibody |
| 中文名稱 | 白細胞介素1受體相關激酶1結合蛋白1抗體 |
| 別 名 | ActA binding protein 3; AIP70; IKBP1_HUMAN; Interleukin 1 receptor associated kinase 1 binding protein 1; IRAK1-binding protein 1; Signaling molecule that associates with Pelle-like kinase; SIMPL. |
| 研究領域 | 細胞生物 信號轉導 激酶和磷酸酶 結合蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 29kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IRAK1BP1: 51-150/260 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
IRAK1BP1 is a component of the IRAK1 dependent TNFRSF1A signaling pathway that leads to NF-kappa-B activation and is required for cell survival. It acts by enhancing RELA transcriptional activity. Function: Component of the IRAK1-dependent TNFRSF1A signaling pathway that leads to NF-kappa-B activation and is required for cell survival. similarity). {ECO:0000250}. Subunit: Interacts with IRAK1 and RELA. Interacts with HSPA8 and HSPA1 (By similarity). {ECO:0000250}. Subcellular Location: Cytoplasmic and Nuclear Post-translational modifications: Phosphorylation at Ser-56 and/or Ser-62 is required for full activity. Phosphorylated on at least one of Ser-235, Thr-237, Ser-242 and Thr-247 upon TNF-alpha activation, which favors nuclear translocation (By similarity). {ECO:0000250}. Similarity: Belongs to the IRAK1BP1 family. {ECO:0000305}. SWISS: Q5VVH5 Gene ID: 134728 Database links: Entrez Gene: 134728 Human Entrez Gene: 65099 Mouse Omim: 615375 Human SwissProt: Q5VVH5 Human SwissProt: Q9ESJ7 Mouse Unigene: 656212 Human Unigene: 41415 Mouse |
| 產品圖片 |
Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti-IRAK1BP1 (bs-16694R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD
Protein: MCF-7(human) lyates at 40ug;
Primary: Rabbit Anti-IRAK1BP1 (bs-16694R) at 1:300;
Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000;
Predicted band size:29 kD
Observed band size:33 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IRAK1BP1 Polyclonal Antibody, Unconjugated(bs-16694R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
