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Rabbit Anti-FBXO44  antibody (bs-16050R)  
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產品編號 bs-16050R
英文名稱 Rabbit Anti-FBXO44  antibody
中文名稱 FBXO44蛋白抗體
別    名 5730411K09; AV001623; DKFZp781J0852; F box gene 3; F box only protein 44; F box only protein 6a; F box protein 44; F box protein FBX30; F box/G domain protein 3; F-box only protein 44; F-box protein FBX30; F-box/G-domain protein 3; FBG3; FBX30; FBX44; FBX44_HUMAN; FBX6A; Fbxo44; Fbxo6a; MGC14140; RGD1562463.  
研究領域 細胞生物  泛素  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Mouse,Rat (predicted: Human,Pig,Sheep,Cow)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 30kDa
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human FBXO44: 101-200/255 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It is also a member of the NFB42 (neural F Box 42 kDa) family, similar to F-box only protein 2 and F-box only protein 6. Four alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function:
Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.

Subunit:
Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with SKP1 and CUL1.

Tissue Specificity:
Abundantly expressed in brain and kidney. Expressed at lower levels in heart, spleen and liver.

Similarity:
Contains 1 F-box domain.
Contains 1 FBA (F-box associated) domain.

SWISS:
Q9H4M3

Gene ID:
93611

Database links:

Entrez Gene: 93611 Human

Entrez Gene: 230903 Mouse

Entrez Gene: 500587 Rat

Omim: 609111 Human

SwissProt: Q9H4M3 Human

SwissProt: Q8BK26 Mouse

Unigene: 556006 Human

Unigene: 220327 Mouse

Unigene: 401783 Mouse



產品圖片
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FBXO44) Polyclonal Antibody, Unconjugated (bs-16050R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FBXO44) Polyclonal Antibody, Unconjugated (bs-16050R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FBXO44) Polyclonal Antibody, Unconjugated (bs-16050R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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