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Rabbit Anti-Elastase-2B  antibody (bs-15598R)  
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產品編號 bs-15598R
英文名稱 Rabbit Anti-Elastase-2B  antibody
中文名稱 胰腺彈性蛋白酶2B抗體
別    名 CEL2B_HUMAN; Chymotrypsin-like elastase family member 2B; Elastase-2B.  
研究領域 細胞生物  新陳代謝  泛素  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 26kDa
細胞定位 細胞外基質 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Elastase-2B 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3A has little elastolytic activity. Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3A preferentially cleaves proteins after alanine residues. Elastase 3A may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1. [provided by RefSeq, Jul 2008]

Function:
Acts upon elastin.

Subcellular Location:
Secreted.

Tissue Specificity:
Pancreas.

Similarity:
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain.

SWISS:
P08218

Gene ID:
51032

Database links:

Entrez Gene: 51032 Human

SwissProt: P08218 human 



產品圖片
Paraformaldehyde-fixed, paraffin embedded (human kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Elastase-2B) Polyclonal Antibody, Unconjugated (bs-15598R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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