| 產品編號 | bs-14544R |
| 英文名稱 | Rabbit Anti-EIF3S2 antibody |
| 中文名稱 | eIF3β蛋白抗體 |
| 別 名 | eIF-3-beta; eIF3 beta; eIF3 p36; eIF3-beta; eIF3-p36; eIF3i; EIF3I_HUMAN; EIF3S2; EIF3S2, formerly; Eukaryotic translation initiation factor 3 subunit 2; Eukaryotic translation initiation factor 3 subunit 2 beta; Eukaryotic translation initiation factor 3 subunit I; eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD; eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa; eukaryotic translation initiation factor 3, subunit 2, formerly; eukaryotic translation initiation factor 3, subunit I; Predicted protein of HQ2242; PRO2242; TGF beta receptor interacting protein 1; TGF-beta receptor-interacting protein 1; TGFbeta receptor interacting protein 1; TGFbeta receptor-interacting protein 1; TRIP 1; TRIP-1; TRIP1. |
| 研究領域 | 細胞生物 轉運蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat (predicted: Mouse,Rabbit,Pig,Cow,Dog) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 37kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EIF3S2: 161-260/325 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that lead to 80S ribosomal assembly and, ultimately, translation. The eukaryotic initiation factor-3 (eIF3) scaffolding structure is the largest of the eIF complexes and includes eIF3 alpha, eIF3 beta, eIF3 gamma, eIF3 delta, eIF3 epsilon, eIF3 omega, eIF3 eta, all of which function to control the assembly of the 40S ribosomal subunit. Association of eIF3 proteins with the 40S ribosomal subunit stabilizes eIF2-GTP-Met-tRNAiMet complex association and mRNA binding, and promotes dissociation of 80S ribosomes into 40S and 60S subunits, thereby promoting the assembly of the pre-initiation complex. Overexpression of eIF3 proteins is common in several cancers, suggesting a role for eIF3 proteins in tumorigenesis. Function: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Subcellular Location: Cytoplasm. Post-translational modifications: Phosphorylated by TGF-beta type II receptor. Similarity: Belongs to the eIF-3 subunit I family. Contains 5 WD repeats. SWISS: Q13347 Gene ID: 8668 Database links: Entrez Gene: 8668 Human Entrez Gene: 419653 Chicken Entrez Gene: 54709 Mouse Entrez Gene: 100008959 Rabbit Entrez Gene: 447488 Xenopus laevis Entrez Gene: 548498 Xenopus tropicalis Omim: 603911 Human SwissProt: Q13347 Human SwissProt: Q9QZD9 Mouse SwissProt: Q5IH81 Rabbit SwissProt: Q66J51 Xenopus laevis SwissProt: Q5EBE8 Xenopus tropicalis Unigene: 530096 Human Unigene: 250874 Mouse Unigene: 162577 Rat Unigene: 77336 Xenopus laevis |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with EIF3S2 polyclonal antibody, unconjugated (bs-14544R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-EIF3S2 Polyclonal Antibody, Unconjugated(bs-14544R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
