| 產品編號 | bs-14394R |
| 英文名稱 | Rabbit Anti-DNAJC5G antibody |
| 中文名稱 | DNAJC5G蛋白抗體 |
| 別 名 | CSP-gamma; DNJ5G_HUMAN; Cysteine string protein gamma; DnaJ (Hsp40) homolog subfamily C member 5 gamma; DnaJ homolog subfamily C member 5G; Gamma CSP; Gamma cysteine string protein. |
| 研究領域 | 細胞生物 信號轉導 轉運蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 21kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DNAJC5G: 101-189/189 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The DnaJ family is one of the largest of all the chaperone families and has evolved with diverse cellular localization and functions. Members of the DnaJ family are characterized by the presence of the J domain. DnaJ heat shock induced proteins are from the bacterium Escherichia coli and are under the control of the htpR regulatory protein. The DnaJ proteins play a critical role in the HSP 70 chaperone machine by interacting with HSP 70 to stimulate ATP hydrolysis. Proteins of the DnaJ family contain cysteine rich regions that are composed of zinc fingers that form a peptide binding domain responsible for chaperone function. DnaJ proteins are important mediators of proteolysis and are involved in the regulation of protein degradation, exocytosis and endocytosis. Function: Contains 1 J domain. Subcellular Location: Membrane; Lipid anchor Tissue Specificity: Testis specific. Post-translational modifications: Palmitoylated. Similarity: Contains 1 J domain. SWISS: Q8N7S2 Gene ID: 285126 Database links: Entrez Gene: 285126 Human GenBank: NP_775921.1 Human Omim: 613946 Human SwissProt: Q8N7S2 Human |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DNAJC5G) Polyclonal Antibody, Unconjugated (bs-14394R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DNAJC5G) Polyclonal Antibody, Unconjugated (bs-14394R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
