| 產品編號 | bs-14233R |
| 英文名稱 | Rabbit Anti-DDX52 antibody |
| 中文名稱 | ATP依賴RNA解旋酶DDX52抗體 |
| 別 名 | ATP dependent RNA helicase ROK1 like; ATP-dependent RNA helicase ROK1-like; DDX52; DDX52_HUMAN; DEAD (Asp-Glu-Ala-Asp) box polypeptide 52; DEAD box protein 52; Probable ATP dependent RNA helicase DDX52; Probable ATP-dependent RNA helicase DDX52; ROK1. |
| 研究領域 | 發育生物學 轉錄調節因子 細胞分化 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human,Mouse,Rat,Rabbit,Sheep,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 67kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DDX52: 501-599/599 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DEAD box protein 52 (DDX52) is a 599 amino acid protein belonging to the DEAD box helicase family. Localized to the nucleus, DDX52 is phosphorylated by ATM or ATR upon DNA damage. DDX52 contains one helicase ATP-binding domain and one helicase C-terminal domain Subcellular Location: Nucleus > nucleolus. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the DEAD box helicase family. DDX52/ROK1 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. SWISS: Q9Y2R4 Gene ID: 11056 Database links: Entrez Gene: 11056 Human Entrez Gene: 78394 Mouse SwissProt: Q9Y2R4 Human SwissProt: Q8K301 Mouse Unigene: 51117 Cow Unigene: 590937 Human Unigene: 280544 Mouse Unigene: 18605 Rat |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
