| 產(chǎn)品編號 | bs-14330R |
| 英文名稱 | Rabbit Anti-DDX49 antibody |
| 中文名稱 | ATP依賴RNA解旋酶DDX49抗體 |
| 別 名 | DEAD (Asp Glu Ala Asp) box polypeptide 49; DEAD box protein 49; DDX49_HUMAN; FLJ10432; Probable ATP dependent RNA helicase DDX49; R27090 2. |
| 研究領(lǐng)域 | 細胞生物 發(fā)育生物學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 結(jié)合蛋白 細胞分化 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Pig,Sheep,Cow,Dog) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 45kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DDX49: 351-483/483 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
DEAD-box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome/spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis and cellular growth and division. DDX49 (DEAD-box protein 49) is a 483 amino acid protein that contains one helicase ATP-binding domain and one helicase C-terminal domain. One of several members of the DEAD-box protein family, DDX49 may function as a an RNA helicase that is involved in pre-mRNA splicing events. Function: The function of DDX49 has not yet been determined. Similarity: Belongs to the DEAD box helicase family. DDX49/DBP8 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. SWISS: Q9Y6V7 Gene ID: 54555 Database links: Entrez Gene: 54555 Human Entrez Gene: 234374 Mouse SwissProt: Q9Y6V7 Human SwissProt: Q3UTV0 Mouse SwissProt: Q4FZF3 Mouse Unigene: 143187 Human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DDX49) Polyclonal Antibody, Unconjugated (bs-14330R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DDX49) Polyclonal Antibody, Unconjugated (bs-14330R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
