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Rabbit Anti-DDX28  antibody (bs-14224R)  
~~~促銷代碼KT202411~~~
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產品編號 bs-14224R
英文名稱 Rabbit Anti-DDX28  antibody
中文名稱 ATP依賴RNA解旋酶DDX28抗體
別    名 DDX 28; DDX28; DDX28_HUMAN; DEAD (Asp Glu Ala Asp) box polypeptide 28; DEAD box polypeptide 28; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 28; FLJ11282; MDDX 28; MDDX28; Mitochondrial DEAD box protein 28.  
研究領域 細胞生物  轉錄調節因子  結合蛋白  線粒體  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Mouse,Rat (predicted: Human,Rabbit,Sheep,Cow,Dog)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 60kDa
細胞定位 細胞核 線粒體
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human DDX28: 451-540/540 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is intronless. It encodes an RNA-dependent ATPase. The encoded protein is localized in the mitochondria and the nucleus, and can be transported between the mitochondria and the nucleus. [provided by RefSeq, Jul 2008]

Function:
May be involved in RNA processing or transport. Has RNA and Mg(2+)-dependent ATPase activity.

Subcellular Location:
Nucleus. Mitochondrion. Transported between these two compartments. Nuclear localization depends on active RNA polymerase II transcription.

Tissue Specificity:
Expressed in all tissues tested, including brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, leukocytes, colon, small intestine, ovary and prostate.

Similarity:
Belongs to the DEAD box helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.

SWISS:
Q9NUL7

Gene ID:
55794

Database links:

Entrez Gene: 55794 Human

Omim: 607618 Human

SwissProt: Q9NUL7 Human

nigene: 458313 Human



產品圖片
Sample: testis (Mouse) Lysate at 40 ug Primary: Anti-DDX28(bs-14224R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min; Incubation: Anti-DDX28 Polyclonal Antibody, Unconjugated(bs-14224R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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