| 產品編號 | bs-14057R |
| 英文名稱 | Rabbit Anti-CRIP2 antibody |
| 中文名稱 | 富含半胱氨酸CRIP2蛋白抗體 |
| 別 名 | CRIP 2; CRIP; CRIP2; CRIP2_HUMAN; CRP 2; CRP; CRP-2; CRP2; Cysteine rich intestinal protein; Cysteine rich protein 2; Cysteine-rich protein 2; ESP 1; ESP1; ESP1 protein; Hlp; Protein ESP1. |
| 研究領域 | 心血管 發(fā)育生物學 轉運蛋白 結合蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 22kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CRIP2: 17-120/208 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a putative transcription factor with two LIM zinc-binding domains. The encoded protein may participate in the differentiation of smooth muscle tissue. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] Tissue Specificity: Widespread tissue expression; highest levels in the heart. Similarity: Contains 2 LIM zinc-binding domains. SWISS: P52943 Gene ID: 1397 Database links: Entrez Gene: 1397 Human Entrez Gene: 68337 Mouse Omim: 601183 Human SwissProt: P52943 Human SwissProt: Q9DCT8 Mouse Unigene: 534309 Human Unigene: 70327 Human Unigene: 133825 Mouse Unigene: 4267 Rat |
| 產品圖片 |
Sample: lung (mouse) Lysate at 40 ug
Primary: Anti-CRIP2(bs-14057R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 30 kD
Paraformaldehyde-fixed, paraffin embedded (mouse embryo); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRIP2) Polyclonal Antibody, Unconjugated (bs-14057R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
