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Rabbit Anti-UBXN2B  antibody (bs-13680R)  
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產(chǎn)品編號 bs-13680R
英文名稱 Rabbit Anti-UBXN2B  antibody
中文名稱 UBXN2B蛋白抗體
別    名 NSFL1 cofactor p37; p37; p97 cofactor p37; UBX domain containing protein 2B; UBX domain protein 2B; UBX domain-containing protein 2B; UBX2B_HUMAN; UBXN2B  
研究領(lǐng)域 細(xì)胞生物  信號轉(zhuǎn)導(dǎo)  泛素  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Mouse,Rat (predicted: Human,Rabbit,Pig,Cow,Dog)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 37kDa
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human UBXN2B: 201-300/331 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The UBX (Ubiquitin regulatory X) domain is an 80 amino acid motif that is usually present on the carboxy-terminus of certain eukaryotic proteins. UBX domain-containing proteins (UBXD), such as FAF1, p33ING1 and D8S2298E, are typically involved in ubiquitin-related processes. UBXD proteins also constitute the largest family of VCP cofactors and are generally involved in substrate recruitment to VCP, as well as regulation of its activity. UBXD2B (UBX domain-containing protein 2B), also known as NSFL1 cofactor p37 and p97 cofactor p37, is a 331 amino acid protein that contains one UBX domain and one SEP domain. UBXN2B is required for ER and Golgi biogenesis and also plays a role in their maintenance during interphase, as well as their reassembly at the end of mitosis. Through interaction with VCP, UBXN2B forms a complex that has membrane fusion activity.

Function:
Adapter protein required for Golgi and endoplasmic reticulum biogenesis. Involved in Golgi and endoplasmic reticulum maintenance during interphase and in their reassembly at the end of mitosis. The complex formed with VCP has membrane fusion activity; membrane fusion activity requires USO1-GOLGA2 tethering and BET1L. VCPIP1 is also required, but not its deubiquitinating activity.

Subunit:
Interacts with VCP. Does not bind ubiquitin (By similarity).

Subcellular Location:
Nucleus. Cytoplasm; cytosol. Endoplasmic reticulum. Golgi apparatus.

Similarity:
Belongs to the NSFL1C family.
Contains 1 SEP domain.
Contains 1 UBX domain.

SWISS:
Q14CS0

Gene ID:
137886

Database links:

Entrez Gene: 137886 Human

Omim: 610686 Human

SwissProt: Q14CS0 Human

Unigene: 155572 Human



產(chǎn)品圖片
Sample: Adrenal gland (Mouse) Lysate at 40 ug Primary: Anti-UBXN2B(bs-13680R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 37 kD
Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UBXN2B) Polyclonal Antibody, Unconjugated (bs-13680R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UBXN2B) Polyclonal Antibody, Unconjugated (bs-13680R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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