| 產(chǎn)品編號 | bs-13663R |
| 英文名稱 | Rabbit Anti-phospho-SLP76 (Tyr113) antibody |
| 中文名稱 | 磷酸化淋巴細胞胞漿蛋白2抗體 |
| 別 名 | SLP76 (phospho Y113); p-SLP76 (Y113); p-LCP2; 76 kDa tyrosine phosphoprotein; CG8697; LCP 2; LCP 2; LCP2; LCP2_HUMAN; Lymphocyte Cytosolic Protein 2; Lymphocyte Cytosolic Protein 2; SH2 Domain Containing Leukocyte Protein 76 KD; SH2 domain containing leukocyte protein of 76kD; SH2 domain containing leukocyte protein of 76kDa; SH2 domain-containing leukocyte protein of 76 kDa; SLP 76; SLP 76; SLP 76 tyrosine phosphoprotein; SLP-76 tyrosine phosphoprotein; SLP76; SLP76 tyrosine phosphoprotein. |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導(dǎo) 激酶和磷酸酶 淋巴細胞 t-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rabbit,Pig,Sheep,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 75kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human SLP76 around the phosphorylation site of Tyr113: DD(p-Y)ES |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The translational product of the Vav proto-oncogene is exclusively expressed in cells of hematopoietic origin and is critical for lymphocyte development and activation. However, the biochemical basis of Vav’s function is unclear. Vav contains a single SH2 domain that is required for its association with the T cell receptor (TCR). Overexpression of Vav or SLP-76 in Jurkat cells leads to NFAT activation and IL-2 production. When co-expressed, Vav and SLP-76 synergize to induce a robust basal and TCR-mediated IL-2 response. Although SLP-76 does not contain a motif that would indicate it to be a member of the tyrosine, serine/threonine or lipid kinase families, it does contain several putative SH2/SH3-binding domains and has been shown to physically associate with the adapter protein GRB2 as well as PLC g1. The discovery of SLP-76 represents an important step in elucidating the mechanism of Vav transformation and TCR-mediated NFAT activation. Function: Involved in T-cell antigen receptor mediated signaling. Subunit: Interacts with SLA. Interacts with CBLB (By similarity). Interacts with the adapter proteins GRB2 and FYB. Interacts with SHB. Interacts with PRAM1. Subcellular Location: Cytoplasm. Tissue Specificity: Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines. Post-translational modifications : Phosphorylated after T-cell receptor activation by ZAP-70. Similarity: Contains 1 SAM (sterile alpha motif) domain. Contains 1 SH2 domain. SWISS: Q13094 Gene ID: 3937 Database links: Entrez Gene: 3937 Human Omim: 601603 Human SwissProt: Q13094 Human Unigene: 304475 Human |
| 產(chǎn)品圖片 |
Sample:
Jurkat(Human) Cell Lysate at 30 ug
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-phospho-SLP76 (Tyr113) (bs-13663R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 75 kD
Observed band size: 75 kD
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