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Rabbit Anti-HINT1  antibody (bs-10228R)  
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產品編號 bs-10228R
英文名稱 Rabbit Anti-HINT1  antibody
中文名稱 組氨酸三聚體核苷結合蛋白1抗體
別    名 histidine triad nucleotide binding protein 1; Adenosine 5' monophosphoramidase; Adenosine 5'-monophosphoramidase; HINT 1; HINT-1; histidine triad nucleotide binding protein 1; histidine triad nucleotide binding protein; histidine triad nucleotide-binding protein; Histidine triad nucleotide-binding protein 1; PKCI 1; PKCI-1; PKCI1; PRKCNH1; Protein kinase C inhibitor 1; Protein kinase C interacting protein 1; Protein kinase C-interacting protein 1; HINT1_HUMAN.  
研究領域 腫瘤  細胞生物  免疫學  結合蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Dog)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 14kDa
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human HINT1: 41-126/126 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The protein encoded by this gene can hydrolyze substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif, which is part of the loop that binds to the substrate. This gene has been found to be a tumor suppressing gene. Several transcript variants, but only one of them protein-coding, have been found for this gene. [provided by RefSeq, Dec 2012].

Function:
Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.

Subunit:
Homodimer. Interacts with CDK7.

Subcellular Location:
Cytoplasm. Nucleus. Note=Interaction with CDK7 leads to a more nuclear localization.

Tissue Specificity:
Widely expressed.

Similarity:
Belongs to the HINT family.
Contains 1 HIT domain.

SWISS:
P49773

Gene ID:
3094

Database links:

Entrez Gene: 3094 Human

Entrez Gene: 15254 Mouse

Entrez Gene: 690660 Rat

Omim: 601314 Human

SwissProt: P49773 Human

SwissProt: P70349 Mouse

SwissProt: P62959 Rat

Unigene: 483305 Human

Unigene: 425 Mouse

Unigene: 138667 Rat



產品圖片
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HINT1) Polyclonal Antibody, Unconjugated (bs-10228R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HINT1) Polyclonal Antibody, Unconjugated (bs-10228R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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