| 產品編號 | bs-13260R |
| 英文名稱 | Rabbit Anti-GABA Transporter 3 antibody |
| 中文名稱 | γ氨基丁酸運載蛋白3抗體 |
| 別 名 | GABT3; GAT-3; GAT3; S6A11_HUMAN; Slc6a11; Sodium- and chloride-dependent GABA transporter 3; SOLUTE CARRIER FAMILY 6 (NEUROTRANSMITTER TRANSPORTER, GABA), MEMBER 11; Solute carrier family 6 member 11. |
| 研究領域 | 細胞生物 神經生物學 轉運蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat) |
| 產品應用 | WB=1:500-2000,Flow-Cyt=1ug/test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 71kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GABA Transporter 3/GAT 3: 531-632/632 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABAergic neurotransmission is terminated by the uptake of GABA into the presynaptic terminal and the surrounding astroglial cells by sodium-dependent transporters, such as SLC6A11 (Borden et al., 1994 [PubMed 7874447]).[supplied by OMIM, Nov 2010] Function: Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Widespread distribution in the brain. Similarity: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A11 subfamily. SWISS: P48066 Gene ID: 6538 Database links: Entrez Gene: 6538 Human Omim: 607952 Human SwissProt: P48066 Human Unigene: 657405 Human |
| 產品圖片 |
Sample:A431 (human)cell Lysate at 40 ug
Primary: Anti-GABA Transporter 3 (bs-13260R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 71 kD
Observed band size: 71 kD
Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-GABA Transporter 3 antibody (bs-13260R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
