| 產品編號 | bs-13044R |
| 英文名稱 | Rabbit Anti-DZIP3 antibody |
| 中文名稱 | E3泛素蛋白連接酶dzip3抗體 |
| 別 名 | DAZ-interacting protein 3; E3 ubiquitin-protein ligase DZIP3; hRUL138; KIAA0675; RNA-binding ubiquitin ligase of 138 kDa; DZIP3_HUMAN. |
| 研究領域 | 細胞生物 表觀遺傳學 泛素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Cow,Dog,Recombinant Fragment,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=2ug/Test,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 139kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DZIP3: 451-550/1208 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). DZIP3 (DAZ interacting protein 3, zinc finger), also known as UURF2 or hRUL138, is a 1,208 amino acid protein that localizes to the cytoplasm and contains one RING-type zinc finger. Expressed in a variety of tissues with highest expression in heart, skeletal muscle and kidney, DZIP3 functions as an E3 ubiquitin-protein ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme, thereby playing a role in signaling events throughout the cell. Multiple isoforms of DZIP3 exist due to alternative splicing events. Function: E3 Ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Able to specifically bind RNA. Subunit: Interacts with DAZ proteins. Subcellular Location: Cytoplasm. Tissue Specificity: Widely expressed at low level. Highlyexpressed in skeletal muscle, kidney and heart. Expressed at lowlevel in placenta, lung, brain, liver and pancreas. Similarity: Contains 1 RING-type zinc finger. SWISS: Q86Y13 Gene ID: 9666 Database links: Entrez Gene: 9666 Human SwissProt: Q86Y13 Human |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
