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Rabbit Anti-DAPP1  antibody (bs-12982R)  
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產(chǎn)品編號 bs-12982R
英文名稱 Rabbit Anti-DAPP1  antibody
中文名稱 B淋巴細(xì)胞銜接分子DAPP1抗體
別    名 B cell adapter molecule of 32 kDa; B lymphocyte adapter protein Bam32; B-cell adapter molecule of 32 kDa; BAM32; DAPP1; DAPP1_HUMAN; DKFZp667E0716; Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide; Dual adaptor of phosphotyrosine and 3 phosphoinositides; hDAPP1.  
Specific References  (1)     |     bs-12982R has been referenced in 1 publications.
[IF=3.69] Honghe Xiao et al. Shenzao jiannao oral liquid, an herbal formula, ameliorates cognitive impairments by rescuing neuronal death and triggering endogenous neurogenesis in AD-like mice induced by a combination of Aβ42 and scopolamine. J Ethnopharmacol. 2020 Sep 15;259:112957.  WB ;  Mouse.  
研究領(lǐng)域 心血管  細(xì)胞生物  神經(jīng)生物學(xué)  淋巴細(xì)胞  b-淋巴細(xì)胞  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Rat (predicted: Human,Mouse,Sheep,Dog,Horse)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 32kDa
細(xì)胞定位 細(xì)胞漿 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human DAPP1/BAM32: 51-160/280 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 B cell adapter molecule is also designated dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide (DAPP1) or B lymphocyte adapter protein. BAM32 is a B cell-associated adapter that is crucial for B cell antigen receptor signaling regulation. BAM32 interacts with Ptdlns and PLC g2 and, upon B cell activation, the protein is phosphorylated on tyrosine residues. It is a mainly cytoplasmic protein that can translocate to the cell membrane after cell stimulation. BAM32, which contains one PH domain and one SH2 domain, is primarily expressed in placenta and lung tissues, but can also be detected in heart, liver, pancreas and brain.

Function:
May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.

Subunit:
Interacts with PtdIns(3,4,5)P3 and PLCG2. In vitro, interacts with PtdIns(3,4)P2.

Subcellular Location:
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.

Tissue Specificity:
Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.

Post-translational modifications:
Phosphorylated on tyrosine residues.

Similarity:
Contains 1 PH domain.
Contains 1 SH2 domain.

SWISS:
Q9UN19

Gene ID:
27071

Database links:

Entrez Gene: 533156 Cow

Entrez Gene: 27071 Human

Entrez Gene: 26377 Mouse

Entrez Gene: 362046 Rat

Omim: 605768 Human

SwissProt: Q9UN19 Human

SwissProt: Q9QXT1 Mouse



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DAPP1) Polyclonal Antibody, Unconjugated (bs-12982R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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