| 產品編號 | bs-10144R |
| 英文名稱 | Rabbit Anti-CD35 antibody |
| 中文名稱 | Ⅰ型補體受體（C3b/C4b受體）抗體 |
| 別 名 | C3 binding protein; C3b/C4b receptor; C3BR; C4BR; CD 35; CD35 antigen; complement component (3b/4b) receptor 1 (Knops blood group); complement component (3b/4b) receptor 1 including Knops blood group system; Complement component receptor 1; Complement receptor 1; Complement receptor type 1; CR 1; CR1; CR1_HUMAN; KN; Knops blood group antigen. |
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Specific References (2) | bs-10144R has been referenced in 2 publications.
[IF=7.097] Jinyang Li. et al. A pentapeptide enabled AL3810 liposome-based glioma-targeted therapy with immune opsonic effect attenuated. Acta Pharm Sin B. 2021 Jan;11:283 IF ; Mouse.
[IF=5.23] Rovituso, Damiano M., et al. "CEACAM1 mediates B cell aggregation in central nervous system autoimmunity." Scientific Reports 6 (2016): 29847. IHC-P ; Mouse.
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| 研究領域 | 免疫學 細胞膜受體 淋巴細胞 t-淋巴細胞 b-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 274kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CR1/CD35: 1301-1400/2039 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The genome is polymorphic at this locus with allele-specific splice variants encoding different isoforms, based on the presence/absence of long homologous repeats (LHRs). The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in this gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus, sarcoidosis and Alzheimer's disease. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. [provided by RefSeq, May 2020] Function: Mediates cellular binding of particles and immune complexes that have activated complement. Subunit: Monomer. Subcellular Location: Membrane; Single-pass type I membrane protein. Similarity: Present on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. Similarity : Belongs to the receptors of complement activation (RCA) family. Contains 30 Sushi (CCP/SCR) domains. SWISS: P17927 Gene ID: 1378 Database links: Entrez Gene: 1378 Human Omim: 120620 Human SwissProt: P17927 Human Unigene: 334019 Human CD35廣泛分布于紅細胞、粒細胞、單核細胞、肥大細胞、濾泡樹突狀細胞、腎小球足突細胞、B細胞及部分CD4+T細胞表面.CD35抗原又稱為CR1或者C3b/C4b R，是一種分子量160-250kDa的C3b/C4b補體結合跨膜蛋白，主要用于濾泡樹突狀細胞標記和濾泡樹突狀細胞瘤的診斷。 |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD35) Polyclonal Antibody, Unconjugated (bs-10144R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: Rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD35 Polyclonal Antibody, Unconjugated(bs-10144R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
