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Rabbit Anti-phospho-APC1 (Ser688)  antibody (bs-12482R)  
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產品編號 bs-12482R
英文名稱 Rabbit Anti-phospho-APC1 (Ser688)  antibody
中文名稱 磷酸化細胞周期末期促進復合蛋白APC1抗體
別    名 phospho-ANAPC1(Ser688); Apc1 (phospho S688); ANAPC 1; ANAPC1; Anaphase promoting complex subunit 1; anaphase-promoting complex 1 (meiotic checkpoint regulator); Anaphase-promoting complex subunit 1; Apc 1; APC1; APC1_HUMAN; Cyclosome subunit 1; MCPR; Meiotic checkpoint regulator; Mitotic checkpoint regulator; Protein Tsg 24; Protein Tsg24; Testis-specific gene 24 protein; TSG 24; TSG24.  
產品類型 磷酸化抗體 
研究領域 細胞生物  免疫學  信號轉導  泛素  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Rat (predicted: Human,Mouse,Chicken)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 215kDa
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human APC1 around the phosphorylation site of Ser688: SL(p-S)PV 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a subunit of the anaphase-promoting complex. This complex is an E3 ubiquitin ligase that regulates progression through the metaphase to anaphase portion of the cell cycle by ubiquitinating proteins which targets them for degradation. [provided by RefSeq, Dec 2011].

Function:
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Subunit:
The APC/C is composed of at least 12 subunits.

Post-translational modifications:
Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis.

Similarity:
Belongs to the APC1 family.
Contains 4 PC repeats.

SWISS:
Q9H1A4

Gene ID:
64682

Database links:

Entrez Gene: 64682 Human

Entrez Gene: 535565 Cow

Entrez Gene: 475750 Dog

Entrez Gene: 17222 Mouse

Omim: 608473 Human

SwissProt: E1BAB6 Cow

SwissProt: E2RLM2 Dog

SwissProt: Q9H1A4 Human



產品圖片
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ANAPC) Polyclonal Antibody, Unconjugated (bs-12482R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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