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Rabbit Anti-DDX23  antibody (bs-12199R)  
~~~促銷(xiāo)代碼KT202411~~~
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產(chǎn)品編號(hào) bs-12199R
英文名稱(chēng) Rabbit Anti-DDX23  antibody
中文名稱(chēng) 三磷酸腺苷依賴(lài)解旋酶DDX23抗體
別    名 100 kDa U5 snRNP-specific protein; DEAD box protein 23; EC 3.6.1.-; PRP28 homolog; U5-100kD; DDX23_HUMAN.  
Specific References  (1)     |     bs-12199R has been referenced in 1 publications.
[IF=6.304] Heng Yang. et al. Therapeutic potential of targeting membrane-spanning proteoglycan SDC4 in hepatocellular carcinoma. Cell Death Dis. 2021 May;12(5):1-16  IF ;  Human.  
研究領(lǐng)域 細(xì)胞生物  細(xì)胞分化  表觀(guān)遺傳學(xué)  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
交叉反應(yīng) (predicted: Human,Mouse,Rat,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 96kDa
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human DDX23: 201-300/820 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 DDX23 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined.

Function:
Involved in pre-mRNA splicing and its phosphorylated form (by SRPK2) is required for spliceosomal B complex formation.

Subunit:
The phosphorylated form (by SRPK2) associates wth tri-snRNP (U4/U6-U5 tri-small nuclear ribonucleoproteins). Identified in the spliceosome C complex. Interacts with ERBB4.

Subcellular Location:
Nuclear

Post-translational modifications:
In vitro phosphorylated by CLK1 and U1 snRNP-associated protein kinase. Phosphorylated by SRPK2 and this phosphorylation is required for its association with the tri-snRNP (U4/U6-U5 tri-small nuclear ribonucleoproteins) and subsequent spliceosomal B complex formation. [SIMILARITY] Belongs to the DEAD box helicase family. DDX23/PRP28 subfamily.

Similarity:
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.

SWISS:
Q9BUQ8

Gene ID:
9416

Database links:

Entrez Gene: 9416 Human

Entrez Gene: 74351 Mouse

Entrez Gene: 300208 Rat

Entrez Gene: 334283 Zebrafish

Omim: 612172 Human

SwissProt: Q9BUQ8 Human

Unigene: 130098 Human



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