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Rabbit Anti-Ctip1  antibody (bs-11544R)  
~~~促銷代碼KT202411~~~
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產品編號 bs-11544R
英文名稱 Rabbit Anti-Ctip1  antibody
中文名稱 B淋巴細胞白血病/淋巴瘤11/鋅指蛋白856抗體
別    名 BCL-11A; B cell CLL/lymphoma 11A (zinc finger protein); B cell CLL/lymphoma 11A (zinc finger protein) isoform 2; B cell CLL/lymphoma 11A; B cell lymphoma/leukaemia 11A; B-cell CLL/lymphoma 11A; B-cell lymphoma/leukemia 11A; BC11A_HUMAN; BCL 11A; BCL-11A; BCL11A; BCL11A B cell CLL/lymphoma 11A (zinc finger protein) isoform 1; BCL11A L; BCL11A S; BCL11A XL; BCL11AL; BCL11AS; BCL11AXL; C2H2 type zinc finger protein; COUP TF interacting protein 1; COUP-TF-interacting protein 1; Ctip 1; CTIP1; Ecotropic viral integration site 9; Ecotropic viral integration site 9 homolog; Ecotropic viral integration site 9 protein; Ecotropic viral integration site 9 protein homolog; Evi 9; EVI-9; Evi9; FLJ10173; FLJ34997; HBFQTL5; KIAA1809; Zinc finger protein 856; ZNF856.  
研究領域 腫瘤  轉錄調節因子  淋巴細胞  b-淋巴細胞  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Mouse,Rat (predicted: Human,Rabbit,Chicken)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 91kDa
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Ctip1: 751-835/835 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, through its interaction with BCL6. During hematopoietic cell differentiation, this gene is down-regulated. It is possibly involved in lymphoma pathogenesis since translocations associated with B-cell malignancies also deregulates its expression. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].

Function:
Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis, is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1.

Subunit:
Interacts with TFCOUP1, PIAS3, ARP1 and EAR2

Subcellular Location:
Cytoplasm. Nucleus. Associates with the nuclear body. Colocalizes with SUMO1 and SENP2 in nuclear speckles.

Tissue Specificity:
Expressed at high levels in brain, spleen thymus, bone marrow and testis. Expressed in CD34-positive myeloid precursor cells, B-cells, monocytes and megakaryocytes. Expression is tightly regulated during B-cell development.

Post-translational modifications:
Sumoylated by SUMO1.

DISEASE:
Note=Chromosomal aberrations involving BCL11A may be a cause of lymphoid malignancies. Translocation t(2;14)(p13;q32.3) causes BCL11A deregulation and amplification.

Similarity:
Contains 6 C2H2-type zinc fingers.

SWISS:
Q9H165

Gene ID:
53335

Database links:

Entrez Gene: 53335 Human

Entrez Gene: 14025 Mouse

Omim: 606557 Human

SwissProt: Q9H165 Human

SwissProt: Q9QYE3 Mouse

Unigene: 370549 Human

Unigene: 441102 Mouse

Unigene: 471050 Mouse

Unigene: 53687 Mouse

Unigene: 88824 Mouse



產品圖片
Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-Ctip1 (bs-11544R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 91 kD Observed band size: 91 kD
Sample: Thymus (Mouse) Lysate at 40 ug Primary: Anti-Ctip1 (bs-11544R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 91 kD Observed band size: 91 kD
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ctip1) Polyclonal Antibody, Unconjugated (bs-11544R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ctip1) Polyclonal Antibody, Unconjugated (bs-11544R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ctip1) Polyclonal Antibody, Unconjugated (bs-11544R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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