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Rabbit Anti-ERR-alpha  antibody (bs-6998R)  
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產品編號 bs-6998R
英文名稱 Rabbit Anti-ERR-alpha  antibody
中文名稱 雌激素受體相關蛋白α抗體
別    名 Estrogen Related Receptor alpha; ERR-α; ERR α; ERRα; ERR alpha; Err 1; ERR a; ERR alpha; ERR-alpha; Err1; ERR1_HUMAN; ERRa; ERRalpha; ESRL 1; ESRL1; ESRR A; Esrra; Estrogen receptor like 1; Estrogen receptor related 1; Estrogen receptor-like 1; Estrogen related receptor alpha; Estrogen-related receptor alpha; Estrra; Nr3b1; Nuclear receptor subfamily 3 group B member 1; Steroid hormone receptor ERR1.  
Specific References  (2)     |     bs-6998R has been referenced in 2 publications.
[IF=3.159] Wan Boyang. et al. Zearalenone promotes follicle development through activating SIRT1/PGC-1α signaling pathway in the ovaries of weaned gilts. J Anim Sci. 2022 Feb;:  WB ;  Pig(Gilt).  
[IF=2.447] Panpan Dong. et al. Roles of ERRα and TGF?β signaling in stemness enhancement induced by 1?μM bisphenol?A exposure via human neural stem cells. Exp Ther Med. 2022 Feb;23(2):1-10  WB ;  Human.  
研究領域 腫瘤  細胞生物  染色質和核信號  信號轉導  生長因子和激素  結合蛋白  細胞骨架  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Mouse (predicted: Rat,Pig,Cow,Dog,Horse)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 47kDa
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Estrogen Related Receptor alpha: 101-200/423 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism.

Subunit:
Binds DNA as a monomer or a homodimer. Interacts (via the AF2 domain) with coactivator PPARGC1A (via the L3 motif); the interaction greatly enhances transriptional activity of genes involved in energy metabolism. Interacts with PIAS4; the interaction enhances sumoylation.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.

SWISS:
P11474

Gene ID:
2101

Database links:

Entrez Gene: 2101 Human

Entrez Gene: 26379 Mouse

Entrez Gene: 293701 Rat

Omim: 601998 Human

SwissProt: P11474 Human

SwissProt: O08580 Mouse

SwissProt: Q5QJV7 Rat

Unigene: 110849 Human

Unigene: 386776 Mouse

Unigene: 130171 Rat



產品圖片
Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-ERR-alpha (bs-6998R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 47 kD
Sample: Lovp(Human) Cell Lysate at 30 ug Primary: Anti- ERR-alpha (bs-6998R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 47 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ERR-alpha) Polyclonal Antibody, Unconjugated (bs-6998R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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