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Goat Anti-Rat IgG H&L antibody (bs-0293G-HRP)

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說明書： 100ul 1ml

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產(chǎn)品編號	bs-0293G-HRP
英文名稱	Goat Anti-Rat IgG H&L antibody
中文名稱	辣根過氧化物酶標(biāo)記的羊抗大鼠IgG H&L
別名	Goat Anti-Rat IgG H&L (HRP); Immunoglobulin G;
	Specific References (19) \| bs-0293G-HRP has been referenced in 19 publications. [IF=14.026] Congcong Chen. et al. Radix Paeoniae Alba attenuates Radix Bupleuri-induced hepatotoxicity by modulating gut microbiota to alleviate the inhibition of saikosaponins on glutathione synthetase. J PHARM ANAL. 2023 Apr;: WB ; Rat. PubMed:10.1016/j.jpha.2023.04.016 [IF=4.051] Zhang, Yamei. et al. miR-18a-5p shuttled by mesenchymal stem cell-derived extracellular vesicles alleviates early brain injury following subarachnoid hemorrhage through blockade of the ENC1/p62 axis. CELL TISSUE RES. 2023 Feb;:1-17 WB ; Rat. PubMed:36795153 [IF=4.011] Wassie T et al. Recombinant B2L and Kisspeptin-54 DNA Vaccine Induces Immunity Against Orf Virus and Inhibits Spermatogenesis In Rats. Sci Rep. 2019 Nov 7;9(1):16262. ELISA ; Rat. PubMed:31700161 [IF=3.947] Ma, Wenbin. et al. CircPCNX Promotes PDGF-BB-Induced Proliferation and Migration of Human Aortic Vascular Smooth Muscle Cells Through Regulating miR-1278/DNMT1 Axis. CARDIOVASC DRUG THER. 2022 Jun;:1-13 WB ; Human. PubMed:35670983 [IF=3.943] Quanyu Chen. et al. Hepatocyte growth factor mediates a novel form of hepatic stem/progenitor cell-induced tolerance in a rat xenogeneic liver rejection model. Int Immunopharmacol. 2021 Jan;90:107180 WB ; Hamster, Rat. PubMed:33221167 [IF=3.503] Zhang-Jie Yang. et al. SIRT 3 was involved in Lycium barbarum seed oil protection testis from oxidative stress: in?vitro and in?vivo analyses. Pharm Biol. 2021;59(1):1314-1325 WB ; Rat. PubMed:10.1080/13880209.2021.1961822 [IF=3.49] Hu, Yong-hua, et al. "Construction and evaluation of a live vaccine against< i> Edwardsiella tarda and< i> Vibrio harveyi: Laboratory vs. mock field trial." Vaccine 29.24 (2011): 4081-4085. ELISA ; Rat. PubMed:21514342 [IF=2.96] Dang, Wei, Min Zhang, and Li Sun. "< i> Edwardsiella tarda DnaJ is a virulence-associated molecular chaperone with immunoprotective potential." Fish & shellfish immunology 31.2 (2011): 182-188. ELISA ; Rat. PubMed:21601637 [IF=2.96] Sun, Yun, Chun-sheng Liu, and Li Sun. "A multivalent killed whole-cell vaccine induces effective protection against< i> Edwardsiella tarda and< i> Vibrio anguillarum." Fish & Shellfish Immunology 31.4 (2011): 595-599. ELISA ; Rat. PubMed:21763772 [IF=2.86] Hou, Shuai, et al. "Mechanism of Mitochondrial Connexin43′ s Protection of the Neurovascular Unit under Acute Cerebral Ischemia-Reperfusion Injury." International Journal of Molecular Sciences 17.5 (2016): 679. WB ; Rat. PubMed:27164087 [IF=2.824] Ling Kong. et al. Tg ROP18 targets IL20RB for host-defense-related-STAT3 activation during Toxoplasma gondii infection. Parasite Vector. 2020 Dec;13(1):1-14 WB ; Monkey. PubMed:32767999 [IF=2.766] Sun and Sun CsBAFF, a Teleost B Cell Activating Factor, Promotes Pathogen-Induced Innate Immunity and Vaccine-Induced Adaptive Immunity. (2015) PLoS.One. 10:e0136015 IF ; Rat. PubMed:26295165 [IF=2.705] Suhaimi H et al. Identification of secretion domain of Neospora caninum profilin. Biochem Biophys Res Commun. 2019 Nov 14. pii: S0006-291X(19)32176-X. WB ; Rat. PubMed:31735333 [IF=2.56] Wang, P. et al. "Attenuation of cytokine production of interleukine -2 and perforin in Two-way mixed lymphocyte reaction by highly efficient transduction of the recombinant adenovirus expressing ICP47 gene."Life Sci J 2013;10(3):963-969 WB ; Rat. PubMed:ISSN:1097-8135 [IF=2.389] Huang Weizhen. et al. LncRNA SNHG11 enhances bevacizumab resistance in colorectal cancer by mediating miR-1207-5p/ABCC1 axis. ANTI-CANCER DRUG. 2022 Jul;33(6):575-586 WB ; Human. PubMed:35324517 [IF=1.88] Wang, Xuguang, and Zhe Chen. "MicroRNA-19a functions as an oncogenic microRNA in non-small cell lung cancer by targeting the suppressor of cytokine signaling 1 and mediating STAT3 activation." International journal of molecular medicine 35.3 (2015): 839-846. WB ; Rat. PubMed:25604748 [IF=1.54] Yuan H et al. Calhex231 alleviates high glucose-induced myocardial fibrosis via inhibiting Itch-ubiquitin proteasome pathway in Vitro. Biol Pharm Bull. 2019 Jun 4. WB ; Rat. PubMed:31167987 [IF=1.291] Iwaki T et al. Nanobody production can be simplified by direct secretion from Escherichia coli. Protein Expr Purif. 2020 Feb 13;170:105607. WB ; Rat. PubMed:32062022 [IF=0] Wang, S. M., et al. "A novel nanobody specific for respiratory surfactant protein A has potential for lung targeting." Clinical, Cosmetic and Investigational Dermatology 8 (2015): 193-204. IHC-F ; Rat. PubMed:25926731
抗體來源	Goat
克隆類型	Polyclonal
交叉反應(yīng)	Mouse (predicted: Rat)
產(chǎn)品應(yīng)用	WB=1:2000-20000,IHC-P=1:100-1000,IHC-F=1:100-1000,ELISA=1:2000-20000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
性狀	Liquid
濃度	2.0 mg/ml
免疫原	Native Rat IgG
亞型	IgG
純化方法	affinity purified by Protein G
緩沖液	10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol.
保存條件	Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng)	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹	Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
產(chǎn)品圖片	Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ly-6G Monoclonal Antibody, Unconjugated(bsm-30178A) at 1:200 overnight at 4°C, followed by conjugation to the bs-0293G-HRP and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ly-6G Monoclonal Antibody, Unconjugated(bsm-30178A) at 1:200 overnight at 4°C, followed by conjugation to the bs-0293G-HRP and DAB (C-0010) staining.

	1、抗體溶解方法
	2、抗體修復(fù)方式
	3、常用試劑的配制
	4、免疫組化操作步驟
	5、免疫組化問題解答
	6、Western Blotting 操作步驟
	7、Western Blotting 問題解答
	8、關(guān)于肽鏈的設(shè)計(jì)
	9、多肽的溶解與保存
	10、酶標(biāo)抗體效價(jià)測定程序

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