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Rabbit Anti-DLK1  antibody (bs-4556R)  
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產(chǎn)品編號(hào) bs-4556R
英文名稱 Rabbit Anti-DLK1  antibody
中文名稱 穿膜蛋白DLK1抗體(C端)
別    名 Adipocyte differentiation inhibitor protein; Brevideltin; Delta like 1 homolog; Delta like homolog; Delta like protein; Delta1; DLK 1; DLK; DLK-1; Dlk1; DLK1_HUMAN; FA 1; FA1; Fetal antigen 1; pG2; Preadipocyte factor 1; Pref 1; Pref; Pref1; Pref-1; Protein delta homolog 1; Secredeltin; ZOG; Delta1; PREF1.  
Specific References  (1)     |     bs-4556R has been referenced in 1 publications.
[IF=2.323] Mengyan Wang. et al. Analysis of the Bovine DLK1 Gene Polymorphism and Its Relation to Lipid Metabolism in Chinese Simmental. Animals-Basel. 2020 Jun;10(6):923  WB ;  Bovine.  
研究領(lǐng)域 腫瘤  細(xì)胞生物  免疫學(xué)  神經(jīng)生物學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  干細(xì)胞  結(jié)合蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse,Rat (predicted: Rabbit,Sheep)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 42kDa
細(xì)胞定位 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from sheep DLK1: 301-381/383 <Cytoplasmic>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a transmembrane protein containing six epidermal growth factor repeats. The protein is involved in the differentiation of several cell types, including adipocytes; it is also thought to be a tumor suppressor. It is one of several imprinted genes located in a region of on chr 14q32. Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). This gene is expressed from the paternal allele. A polymorphism within this gene has been associated with child and adolescent obesity. The mode of inheritance for this polymorphism is polar overdominance; this non-Mendelian inheritance pattern was first described in sheep with the callipyge phenotype, which is characterized by muscle hypertrophy and decreased fat mass. [provided by RefSeq, Mar 2010].

Function:
May have a role in neuroendocrine differentiation. Inhibits adipocyte differentiation.

Subunit:
Monomer.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Tissue Specificity:
Highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary and, to a lesser degree, in adult kidney, muscle, thymus and heart.

Post-translational modifications:
N- and O-glycosylated.

Similarity:
Contains 6 EGF-like domains.

SWISS:
P80370

Gene ID:
8788

Database links:

Entrez Gene: 641301 sheep

Entrez Gene: 281117 Cow

Entrez Gene: 8788 Human

Entrez Gene: 13386 Mouse

Entrez Gene: 114587 Rat

Omim: 176290 Human

SwissProt: P80370 Human

SwissProt: Q09163 Mouse

Unigene: 533717 Human

Unigene: 157069 Mouse

Unigene: 14547 Rat



穿膜蛋白DLK1又稱肝癌相關(guān)基因,是表皮生長(zhǎng)因子(EGF)家族的成員之一,其結(jié)構(gòu)特征為蛋白的細(xì)胞外區(qū)域擁有在結(jié)構(gòu)及氨基酸序列上與表皮生長(zhǎng)因子高度同源的重復(fù)結(jié)構(gòu).DLK1可以抑制脂肪前體細(xì)胞向脂肪細(xì)胞分化,在正常神經(jīng)內(nèi)分泌細(xì)胞及其腫瘤細(xì)胞中表達(dá), DLK1在神經(jīng)內(nèi)分泌軸中起著調(diào)控細(xì)胞生長(zhǎng)分化的作用,同時(shí)DLK1在胚胎肝、造血干細(xì)胞及早期肌肉組織中均有表達(dá)。
產(chǎn)品圖片
Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Rat Cerebrum tissue lysates Primary: Anti-DLK1 (bs-4556R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 45 kDa
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DLK1) Polyclonal Antibody, Unconjugated (bs-4556R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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