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Rabbit Anti-RNASE4  antibody (bs-6583R)  
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產(chǎn)品編號(hào) bs-6583R
英文名稱(chēng) Rabbit Anti-RNASE4  antibody
中文名稱(chēng) 核糖核酸酶4抗體
別    名 RAB1; Ribonuclease 4; Ribonuclease A B1; Ribonuclease, RNase A family, 4; RNAS4_HUMAN; RNase 4; RNS4.  
研究領(lǐng)域 腫瘤  心血管  細(xì)胞生物  信號(hào)轉(zhuǎn)導(dǎo)  血管內(nèi)皮細(xì)胞  細(xì)胞骨架  表觀遺傳學(xué)  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
交叉反應(yīng) Human,Rat (predicted: Mouse,Pig,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 14kDa
細(xì)胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human RNASE4 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The protein encoded by this gene belongs to the pancreatic ribonuclease family. It plays an important role in mRNA cleavage and has marked specificity towards the 3' side of uridine nucleotides. Alternative splicing results in four transcript variants encoding the same protein. This gene and the gene that encodes angiogenin share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2013].

Function:
This RNase has marked specificity towards the 3' side of uridine nucleotides.

Subcellular Location:
Secreted.

Similarity:
Belongs to the pancreatic ribonuclease family.

SWISS:
P34096

Gene ID:
6038

Database links:

Entrez Gene: 6038 Human

Entrez Gene: 58809 Mouse

Entrez Gene: 56759 Rat

SwissProt: P34096 Human

SwissProt: Q9JJH1 Mouse

SwissProt: O55004 Rat



產(chǎn)品圖片
Sample: Lane 1: Human HepG2 cell lysates Lane 2: Human HUVEC cell lysates Primary: Anti-RNASE4 (bs-6583R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 14 kDa Observed band size: 15 kDa
Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-RNASE4/Angiogenin Polyclonal Antibody, Unconjugated(bs-6583R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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