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Rabbit Anti-SIRT7  antibody (bs-5973R)  
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產(chǎn)品編號(hào) bs-5973R
英文名稱 Rabbit Anti-SIRT7  antibody
中文名稱 沉默調(diào)節(jié)樣蛋白SirT7抗體
別    名 NAD dependent deacetylase sirtuin 7; NAD-dependent deacetylase sirtuin-7; Silent mating type information regulation 2 homolog; Silent mating type information regulation 2 S.cerevisiae homolog 7; SIR2 L7; SIR2 like protein 7; Sir2 related protein type 7; SIR2, S.CEREVISIAE, HOMOLOG-LIKE 7; SIR2-like protein 7; SIR2L 7; SIR2L7; SIRT 7; SIR7_HUMAN; Sirtuin 7; Sirtuin type 7; Sirtuin7.  
Specific References  (3)     |     bs-5973R has been referenced in 3 publications.
[IF=3.06] Wronska, A., et al. "Age-Related Changes in Sirtuin 7 Expression in Calorie-Restricted and Refed Rats." Gerontology (2015).  WB ;  
[IF=2.65] Deng Disi. et al. Protective Effect of XinJiaCongRongTuSiZiWan on the Reproductive Toxicity of Female Rats Induced by Triptolide. EVID-BASED COMPL ALT. 2022;2022:3642349  IHC ;  Rat.  
[IF=1.11] Takumida, Masaya, Hiroshi Takumida, and Matti Anniko. "Localization of sirtuins in the mouse inner ear." Acta Oto-Laryngologica 0 (2014): 1-8.  IHC-F ;  Mouse.  
研究領(lǐng)域 腫瘤  心血管  免疫學(xué)  染色質(zhì)和核信號(hào)  信號(hào)轉(zhuǎn)導(dǎo)  細(xì)胞凋亡  轉(zhuǎn)錄調(diào)節(jié)因子  激酶和磷酸酶  結(jié)合蛋白  表觀遺傳學(xué)  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Mouse (predicted: Human,Rat,Pig,Chicken,Dog)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 45kDa
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human SIRT7: 231-330/400 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 SIRT7 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.

Function:
NAD-dependent protein deacetylase. Required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis. Promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex.

Subunit:
Interacts with UBTF and the RNA polymerase I complex. Interacts with histone H2A and/or histone H2B.

Subcellular Location:
Cytoplasm. Nucleus, nucleolus. Note=Located close to the nuclear membrane when in the cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis.

Post-translational modifications:
Phosphorylated during mitosis (Probable).

Similarity:
Belongs to the sirtuin family. Class IV subfamily.
Contains 1 deacetylase sirtuin-type domain.

SWISS:
Q9NRC8

Gene ID:
51547

Database links:

Entrez Gene: 505662 Cow

Entrez Gene: 51547 Human

Entrez Gene: 209011 Mouse

Omim: 606212 Human

SwissProt: Q0P595 Cow

SwissProt: Q9NRC8 Human

SwissProt: Q8BKJ9 Mouse

Unigene: 514636 Human

Unigene: 292957 Mouse



產(chǎn)品圖片
Sample:Small intestine (Mouse)Lysate at 40 ug Primary: Anti-SIRT7(bs-5973R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 45kD Observed band size: 50kD
Sample:Lymph node (Mouse)Lysate at 40 ug Primary: Anti-SIRT7(bs-5973R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 45kD Observed band size: 50kD
Tissue/cell: mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SIRT7 Polyclonal Antibody, Unconjugated(bs-5973R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: mouse splenocytes(blue) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).
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