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Rabbit Anti-CTNNBIP1  antibody (bs-4095R)  
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產(chǎn)品編號(hào) bs-4095R
英文名稱 Rabbit Anti-CTNNBIP1  antibody
中文名稱 β鏈接素相互作用蛋白1抗體
別    名 Beta catenin interacting protein 1; Beta catenin interacting protein ICAT; Catenin beta interacting protein 1; CTNNBIP 1; ICAT; Inhibitor of beta catenin and Tcf 4; MGC15093; CNBP1_HUMAN.  
Specific References  (1)     |     bs-4095R has been referenced in 1 publications.
[IF=6.52] Qi, Wei, et al. "Targeting the Wnt‐regulatory protein CTNNBIP1 by microRNA‐214 enhances the stemness and self‐renewal of cancer stem‐like cells in lung adenocarcinomas." STEM CELLS (2015).  IHC-F ;  Human.  
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  結(jié)合蛋白  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse (predicted: Rat,Rabbit,Cow,Chicken,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 9kDa
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CTNNBIP1: 3-70/81 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 CTNNBIP1 (Catenin, beta interacting protein 1) binds to beta-catenin and prevents the interaction between beta-catenin and TCF transcription factor family members. It is a negative regulator of the Wnt signaling pathway.

Function:
Prevents the interaction between CTNNB1 and TCF family members, and acts as negative regulator of the Wnt signaling pathway.

Subunit:
Binds CTNNB1.

Subcellular Location:
Cytoplasm (By similarity). Nucleus (By similarity).

Similarity:
Belongs to the CTNNBIP1 family.

SWISS:
Q9NSA3

Gene ID:
56998

Database links:

Entrez Gene: 56998 Human

Entrez Gene: 67087 Mouse

Entrez Gene: 503000 Rat

Omim: 607758 Human

SwissProt: Q9NSA3 Human

SwissProt: Q9JJN6 Mouse

Unigene: 463759 Human

Unigene: 299735 Mouse

Unigene: 162132 Rat



產(chǎn)品圖片
Sample: U937(Human) Cell Lysate at 30 ug Primary: Anti- CTNNBIP1 (bs-4095R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 9 kD Observed band size: 9 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CTNNBIP1) Polyclonal Antibody, Unconjugated (bs-4095R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CTNNBIP1) Polyclonal Antibody, Unconjugated (bs-4095R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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